2013 Fiscal Year Final Research Report

Search for chemoattractant of Ralstonia solanacearum in tomato root exudate

Research Project

Project/Area Number	23580146
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Bioproduction chemistry/Bioorganic chemistry
Research Institution	Tokyo University of Agriculture and Technology
Principal Investigator	NATSUME Masahiro 東京農工大学, (連合)農学研究科(研究院), 教授 (10201683)
Project Period (FY)	2011 – 2013
Keywords	青枯病菌 / 走化性 / 根滲出液 / トマト / 誘引物質
Research Abstract	Chemoattractant for Ralstonia solanacearum was searched in the root exudate of tomato. The root exudate of tomato was fractionated into EtOAc-soluble fr. and absorbed and non-absorbed (passed) fr. of activated charcoal column, and their chemoattractive activity was examined. Passed fr. of activated charcoal column showed strongest activity and absorbed fr. was less active. Comparison of chemoattractive activity between passed fr. and amino acid mixture equivalent to the fr. showed the presence of chemoattractant other than amino acids. Fractionated root exudates of non-host plant of R. solanacearum, i.e. rice, rape and alfalfa were prepared and their chemoattractive activity was examined. As a result, passed fr. of activated charcoal column of tomato showed stronger activity than those of other plants.