2013 Fiscal Year Final Research Report
Construction of trichothecene detection system and screening system for detoxifying microorganisms
| Project/Area Number |
23580185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Toyo University |
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Principal Investigator |
ANDO Naoko 東洋大学, 理工学部, 准教授 (70360485)
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| Co-Investigator(Kenkyū-buntansha) |
MINEGISHI Hiroaki 東洋大学, バイオ・ナノエレクトロニクス研究センター, 研究助手 (30440019)
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| Project Period (FY) |
2011 – 2013
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| Keywords | カビ毒 / トリコテセン / 検出系 / 解毒分解 / スクリーニング / 食の安全 |
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| Research Abstract |
Trichothecenes are mycotoxins which are produced by Fusarium and other genera. They contaminate important crops, and can cause serious health problems in humans and livestock. In this research, we found 22 trichothecene resistance genes in Saccharomyces cerevisiae. We constructed multiple gene deletion mutants, and among them, the triple mutant which deleted pdr5, erg6, and rpb4, showed the highest sensitivity toward various trichothecenes. We constructed an effective detective system using disc diffusion test, which allows us to detect deoxynivalenol contaminated with the provisional level in wheat and wheat flower. Next, we screened for microoganisms which can detoxify T-2 toxin, deoxynivalenol and 4-acetylnivalenol. We collected ~10,000 microorganisms mainly from soil, and we found that there were more than 10% microorganisms can deacetylate T-2 toxin and 4-acetyl nivalenol, which resulted in reducing toxicity. However, no organisms can detoxify deoxynivalenol.
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[Journal Article] Development of a highly sensitive yeast bioassay for trichothecene detection2013
Author(s)
Akira TANAKA, Yumi YAMANE, Yohei KOMIYA, Kohta YAMAUCHI, Tomoki SUGIYAMA, Akinobu ECHIGO, Ron USAMI, Yasuhiko YOSHIDA, Fumiyoshi ABE, Hiroaki MINEGISHI, Naoko TAKAHASHI-ANDO
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Journal Title
Mycotoxins
Volume: 63
Pages: 161-170
DOI
Peer Reviewed
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