2013 Fiscal Year Final Research Report
Involvement of s-nitrosylation on the effect of nitric oxide and its production in rat placenta
| Project/Area Number |
23580413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Kazuaki 麻布大学, 獣医学部, 准教授 (50345873)
MORITA Hidetoshi 麻布大学, 獣医学部, 教授 (70257294)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Takehito 麻布大学, 獣医学部, 講師 (90532052)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 一酸化窒素(NO) / S-ニトロシル化 / 子宮・胎盤 / スピントラップ・EPR / ラット |
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| Research Abstract |
We examined the role of nitric oxide (NO) in vascular endothelial growth factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) inhibitor, L-NAME was constantly infused into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO production declined to about 15% of the control level as monitored by NO trapping and electron paramagnetic resonance spectroscopy. VEGF expression was temporally decreased by L-NAME, but recovered to normal levels after 24 h of treatment, whereas hypoxia inducible factor (HIF-1a and inducible NOS (iNOS) expression increased. VEGF expression decreased significantly in placental explants after 6 h of co-treatment with L-NAME and lipopolysaccharide, an iNOS inducer. These results indicate that NO induce VEGF expression in vivo and in vitro in the rat placenta, suggesting that peaked NO production was maintained by a reciprocal relationship between NO and VEGF via HIF-1a.
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Author(s)
Kurihara Y, Suzuki T, Sakaue M, Murayama O, Miyazaki Y, Onuki A, Aoki T, Saito M, Fujii Y, Hisasune M, Tanaka K and Takizawa T.
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Journal Title
J. Vet. Med. Sci
Volume: 76(1)
Pages: 15-23
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[Journal Article] Nitric oxide induces vascular endothelial growth factor expression in the rat placenta in vivo and in vitro2013
Author(s)
Abe H, Ishikawa W, Kushima T, Nishimura T, Mori C, Onuki A, Suzuki T, Ishii Y, Kansaku N, Miyazaki Y, Tanaka K, Morita H and Takizawa T.
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Biosci. Biotechnol. Biochem
Volume: 77(5)
Pages: 971-6
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Suzuki T, Uchida M, Takeda Y, Mori C, Onuki A, Miyazaki Y, Onda K, Ushikoshi S, Shitori K, Tanaka K, Morita H and Takizawa T.
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Journal Title
Biosci. Biotechnol. Biochem
Volume: 77(7)
Pages: 1384-9
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