2013 Fiscal Year Final Research Report
Modification of rattle snake venom derived blood clot dissolver, Alfimeprase, by fusion with C-terminal domain of habu-HR1a and fibrin-targeting peptide
| Project/Area Number |
23590199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Teikyo Heisei University |
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Principal Investigator |
ISHIDA Isao 帝京平成大学, 薬学部, 教授 (00415556)
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| Co-Investigator(Kenkyū-buntansha) |
IKEMOTO Mamoru 帝京平成大学, 薬学部, 准教授 (90311331)
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオテクノロジー / タンパク質 / 酵素 |
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| Research Abstract |
Alfimeprase(A) can digest fibrin directly, and do not produce any plasmin in different from tPA (Activase). Because A can be rapidly trapped by alpha-2 macroglobulin (alfpha-2M) and inactivated in the blood vein, the clinical effect of A in the phase 2 has not been so good to advance its development. We designed the gene in which the C-terminal side of the A was fused with the C-terminal domain (collagen-binding domain) of the HR1a. Considering targeting to fibrin clots, the C-terminal side of the AH gene was fused with the gene for the human plasminogen-derived kringle domain 1 region, which fusion protein is called AHP. The A, AH and AHP genes were inserted into the pE-SUMO vectors and were expressed in the E.coli BL21(DE3)star. Fusion of the HR1a C-teriminal domain with A did not have any effect in terms of avoiding of inactivation by alpha-2M regardless of the presence or absence of the N-terminal SUMO-peptide.
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Research Products
(27 results)
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[Journal Article] Influence of 50-nm polystyrene particles in inducing cytotoxicity in mice co-injected with carbon tetrachloride, cisplatin, or paraquat2012
Author(s)
Shimizu, Y, Isoda, K, Tezuka, E, Yufu, T, Nagai, Y, Ishida, I, Tezuka M
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Journal Title
Pharmazie
Volume: Aug67(8)
Pages: 712-714
Peer Reviewed
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[Journal Article] Characterization of human type monoclonal antibodies against the reduced form of hemin binding protein 35 (HBP35) from Porphyromonas gingivalis2011
Author(s)
Shibata, Y, Okano, S, Shiroza, T, Tahara, T, Nakazawa, K, Katoka, S, Ishida, I, Kobayashi, T, Yoshie, H and Abiko, Y
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Journal Title
J. Periodontal Res
Volume: 46(6)
Pages: 673-681
Peer Reviewed
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[Journal Article] Affinity transfer to human protein by CD3 grafting of camelid VHH2011
Author(s)
Inoue, H, IIhara, A, Takahashi, H, Shimada, I, Ishida, I and Maeda, Y
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Journal Title
Protein Science
Volume: 20
Pages: 1971-1981
Peer Reviewed
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[Journal Article] Fully human monoclonal antibody directed to proteolytic cleavage site in SARS coronavirus S protein neutralizes virus in Rhesus macaque SARS model2011
Author(s)
Miyoshi-Akiyama, T, Ishida, I, Fukushi, M, Yamaguchi, K, Matsuoka, Y, Ishihara, T, Tsukahara, M, Hatakeyama, S, Kurosu, H, Itoh, N, Morisawa, A, Yoshinaka, Y, Yamamoto,N, Lianfeng, Z, Chuan, Q, Kirikae, T and Sasazuki, T
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Journal Title
J Infectious Diseases
Volume: 203, June 1
Pages: 1574-1581
Peer Reviewed
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[Journal Article] Direct generation of induced pluripotent stem cells from human non-mobilized blood2011
Author(s)
Kunisato, A, Wakatsuki, M, Shimba, H, Ohta, T, Ishida, I and Nagao, K
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Journal Title
Stem Cells and Development
Volume: 20(1)
Pages: 159-168
Peer Reviewed
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