Analysis of lipid domains providing platforms for signal transduction

2014 Fiscal Year Final Research Report

• Project/Area Number: 23590234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kagoshima University
• Principal Investigator: AKIKAZU Fujita 鹿児島大学, 共同獣医学部, 教授 (60282232)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 電子顕微鏡 / 脂質 / イノシトールリン脂質 / 脂質ラフト / 凍結活断レプリカ / 糖脂質

Outline of Final Research Achievements

We have developed quick-freezing and freeze-fracture replica labeling (QF-FRL) method to analyze lipid distribution at the nano-meter level. The analysis of lipid distribution was limited to the lipids in the plasma membrane of the cell at first. In this study, we have further developed the QF-FRL method to analyze lipids in the membrane of various organelles at the inside of yeast and mammalian cells. And now we can establish the technique to specifically label phosphatidyl inositol 4-phosphat (PtdIns4P) by using anti-PtdIns4P antibody in the plasma membrane and the membrane of various organelle of inside cell. Now we examine the distribution of PtdIns4P at the nano-scale level.

Free Research Field

細胞生物学