Roles of basement membrane-actin cytoskeletal system on the epithelial branching of embryonic submandibular gland of mouse

2015 Fiscal Year Final Research Report

• Project/Area Number: 23590243
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kitasato University
• Principal Investigator: KADOYA Yuichi 北里大学, 医療衛生学部, 教授 (10185887)
• Project Period (FY): 2011-04-28 – 2016-03-31
• Keywords: 唾液腺 / 分枝形態形成 / 細胞骨格 / 焦点結合 / ライブイメージング

Outline of Final Research Achievements

I attempted to clarify roles of the basement membrane-actin cytoskeleton system for the epithelial branching morphogenesis. Submandibular rudiments from the embryonic day-13 mice were dissected and cultured. Some rudiments were further treated with dispase and the epithelia were separated. Then they were monolayer-cultured on the iMatrix511-coated culture dish, or embedded within the Matrigel. Effects of a myosin II inhibitor, blebbistatin, and a focal adhesion kinase inhibitor, PF573228, were tested in these culture conditions. The phosphorylation of the focus adhesion kinase was found to play roles for the salivary gland branching morphogenesis. A suppressive role of the myosin II was also suggested for controlling the clefting. However, mechanism controlling the initiation and elongation of cleft remained for the future studies.

Free Research Field

解剖学（含む組織学）