Roles of syntaxin1A in the regulation of exocytosis and synaptic vesicle movement in dopaminergic neuron

2013 Fiscal Year Final Research Report

• Project/Area Number: 23590307
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Sophia University
• Principal Investigator: SASAKAWA Nobuyuki 上智大学, 理工学部, 教授 (20187107)
• Project Period (FY): 2011 – 2013
• Keywords: exocytosis / chromaffin cell / amperometry / dopaminergic neuron

Research Abstract

Chromaffin cells were enzymatically dissociated from adrenal medulla from male C57BL mice. By use of amperometry, single exocytotic events were analyzed in single mouse chromaffin cells with a carbon fiber microelectrode. To characterize a single exocytotic event detected as a single current spike, the spike parameters and the frequency of events were analyzed. To clarify the implication of the interaction of syntaxin 1A and CaMKII in the regulation of exocytosis, we examined the events frequency and the kinetics of single events in the cells prepared from R151G syntaxin 1A knock-in mice. The significant reduction was observed in the frequency of exocytotic events evoked by acetylcholine in knock-in cells. The frequency distribution of the spike height, middle width and area were slightly shifted to smaller values in knock-in cells. Syntaxin 1A knock-in seemed to affect not only the event frequency but also the kinetics of the fusion process.

Research Products

• [Journal Article] Involvement of theα1D-adrenergic receptor in methamphetamine-induced hyperthermia and neurotoxicity in rats (2013)
  • Author(s): Kikuchi-Utumi, K, Ishizaka, M., Matsumura, N., Watanabe, M., Aoyama, K., Sasakawa, N., Nakaki, T.
  • Journal Title: Neurotox Res Volume: 80(PMID :23283760) Pages: 2886-2893
  • Peer Reviewed
• [Journal Article] Unique biological activity of botulinum D/C mosaic neurotoxin in murine species (2012)
  • Author(s): Nakamura, K., Kohda, T., Shibata, T., Tsukamoto, K., Arimitsu, H., Hayashi, M., Mukamoto, M., Sasakawa., Kozaki, S.
  • Journal Title: Infect. Immun. Volume: 80(PMID :22665374) Pages: 2886-2893
  • Peer Reviewed
• [Journal Article] Dissociation of inositol- polyphosphates from the C2B domain of synaptotagmine facilitates spontaneous release of catecholamines in adrenal chromaffin cells. A suggestive evidence of a fusion clamp by synaptotagmin (2011)
  • Author(s): Sasakawa, N., Ohara-Imaizumi, M., Fukuda, M., Kobayama, H., Kumakura, K.
  • Journal Title: Neuropharmacol Volume: 60(PMID :21402086) Pages: 1364-1370
  • Peer Reviewed
• [Presentation] Unique biological activity of botulinum D/C mosaic neurotoxin (2012)
  • Author(s): 中村佳司, 幸田知子, 柴田優斗, 塚本健太郎, 有満秀幸, 向本雅郁, 笹川展幸, 小崎俊司
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] Effects of D/C mosaic botulinum neurotoxin on the regulation of exocytosis in single adrenal chromaffin cells (2012)
  • Author(s): 林光紀, 柴田優斗, 中村佳司, 小崎俊司, 笹川展幸
  • Organizer: 第85回日本薬理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-15