2013 Fiscal Year Final Research Report
Multiple functions of ASC that mainly regulates the formation of inflammasome
| Project/Area Number |
23590566
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIDA Shigeaki 信州大学, 医学系研究科, 准教授 (10345762)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ASC / Inflammasome / innate immunity / cell death / cancer / fascin / IL-1beta / IFNbeta |
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| Research Abstract |
ASC responsible for inflammasome fomrmation is involved in both pro-inflammation and pro-apoptosis. We observed the occurrence of the colon cancer was clearly retarded by ASC deletion from Ahr-/-mouse, a prone to colon cancer. In Ahr-/-mice, it was observed that an oncogene beta-catenin was accumulated in nuclei of the intestinal epithelium associated with an increase in the epression of IL-1 1 beta and severe inflammation was suppressed. On one hand, we observed that the metastatic potential of cancer cells were enhanced by ASC knock-down, while Asc over-expression suppressed the survival of cancer cells specifically in the confluent state.
When we searched for the ASC binding proteins by immunoprecipitaiton and mass spectrometer, cytoskeletal and signal transduction-relating molecules, such as Fascine and IQGAP were, and their direct interaction with ASC was confirmed. The investigation of the biological significance of the interaction is underway.
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[Journal Article] Nucleic acid sensing by T cells initiates Th2 cell differentiation2014
Author(s)
Imanishi T, Ishihara C, Badr Mel S, Hashimoto-Tane A, Kimura Y, Kawai T, Takeuchi O, Ishii KJ, Taniguchi S, Noda T, Hirano H, Brombacher F, Barber GN, Akira S, Saito T
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Journal Title
Nat Commun
Volume: 10;5
Pages: 3566
DOI
Peer Reviewed
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[Journal Article] NLRP3 Regulates Neutrophil Functions and Contributes to Hepatic Ischemia-Reperfusion Injury Independently of Inflammasomes2014
Author(s)
Inoue Y, Shirasuna K, Kimura H, Usui F, Kawashima A, Karasawa T, Tago K, Dezaki, Nishimura S, Sagara J, Noda T, IwakuraY, Hiroko Tsutsui H, Taniguchi S,Yanagisawa K, Yada T, Yasuda Y, Takahashi M
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Journal Title
J Immunol
Volume: 192(9)
Pages: 4342-4351
DOI
Peer Reviewed
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[Journal Article] ASC in Renal Collecting Duct Epithelial Cells Contributes to Inflammation and Injury after Unilateral Ureteral Obstruction2014
Author(s)
Komada T, Usui F, Shirasuna K, Kawashima A, Kimura H, Karasawa T, Nishimura S, Sagara J, Noda T, Taniguchi S, Muto S, Nagata D, Kusano E, Takahashi M
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Journal Title
Am J Pathol
Volume: 184(5)
Pages: 1287-1298
DOI
Peer Reviewed
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[Journal Article] Beneficial innate signaling interference for antibacterial responses by a Toll-like receptor-mediated enhancement of the MKP-IRF3 axis2013
Author(s)
Negishi H, Matsuki K, Endo N, Sarashina H, Miki S, Matsuda A, Fukazawa K, Taguchi-Atarashi N, Ikushima H, Yanai H, Nishio J, Honda K, Fujioka Y, Ohba Y, Noda T, Taniguchi S, Nishida E, Zhang Y, Chi H, Flavell RA, Taniguchi T
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Journal Title
Proc Natl Acad Sci USA
Volume: 110(49)
Pages: 19884-19889
DOI
Peer Reviewed
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[Journal Article] ASC-associated inflammation promotes cecal tumorigenesis in aryl hydrocarbon receptor- deficient mice2013
Author(s)
Ikuta T, Kobayashi Y, Kitazawa M, Shiizaki K, Itano N, Noda T, Pettersson S, Poellinger L, Fujii-Kuriyama Y, Taniguchi S, Kawajiri K
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Journal Title
Carcinogenesis
Volume: 34(7)
Pages: 1620-1627
DOI
Peer Reviewed
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