2013 Fiscal Year Final Research Report
Standardization of the measuring method for lupus anticoagulant (LA)
| Project/Area Number |
23590684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
IEKO Masahiro 北海道医療大学, 歯学部, 教授 (50250436)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Nobuhiko 北海道医療大学, 歯学部内科学分野, 准教授 (20372279)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ループスアンチコアグラント / 抗リン脂質抗体 / 血栓症 / 交差混合試験 |
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| Research Abstract |
Standardization of the measuring method for lupus anticoagulant (LA), which is a risk factor of thrombosis, was investigated. The plasma (residual platelets: less than 3,000/uL) obtained by single centrifugation at 1500 x g for 15 minutes, is quite useful for LA tests, because LA activity was significantly changed in plasma with more than 1,200/uL of residual platelets. On the other hand, the sensitivity of APTT reagents for LA is dependent on phospholipid concentration and not the activator, according to our results of LA activity using APTT reagents with same phospholipid condition and silica or ellagic acid as a activator. In an APTT cross-mixing test, 10% and 20% ratio of LA plasma and a 50% ratio of factor VIII deficient plasma or inhibitor positive plasma produced optimum results. Therefore, it is recommended to carry out the mixing test on 5 different mixing ratios of 0, 10, 20, 50, 100% of patient plasma.
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[Journal Article] 交差混合試験における混合比率およびコントロール血漿に関する検討2011
Author(s)
家子正裕, 福武勝幸, 内藤済悦, 吉田美香, 表美香, 静怜子, 安藤秀実, 下村大樹, 西脇圭子, 片岡美香, 山野智子, 中野かおり, 矢島智志, 矢後弘和
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Journal Title
日本検査血液学科雑誌
Volume: 12
Pages: 312-322
Peer Reviewed
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