Research for the mechanism of aldosterone synthesis in the adrenal gland and the heart.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591089
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: YOSHIMURA Michihiro 東京慈恵会医科大学, 医学部, 教授 (30264295)
• Project Period (FY): 2011 – 2013
• Keywords: アルドステロン / 心筋細胞 / 高血糖 / 虚血再灌流障害

Research Abstract

We aimed at the establishment of the experimental system which showed small quantity of aldosterone (Aldo) synthesized in the heart. Various conditions of culture medium and time course were examined using the cultured neonatal rat cardiomyocytes (NRCs). We found that CYP11B2 gene was expressed by short time stimulation of high glucose. This system will be extensively utilizable in the future research of cardiac Aldo synthesis. On the other hand, an unknown action of Aldo was examined by using the Langendorff system. It was found that the pre-treatment of Aldo attenuated ischemia reperfusion injury, suggesting that Aldo per se has a preconditioning action. Also, we newly established the cell culture system which showed the precise intracellular sodium concentration. By this system, we could measure the absolute increase in the intra-cellular sodium concentration stimulated by Aldo and cortisol. This system is also utilizable for the future studies of cardiac hypertrophy and others.

Research Products

• [Journal Article] Preconditioning actions of aldosterone through p38 signaling modulation in isolated rat hearts (2014)
  • Author(s): Yoshino T, Nagoshi T, Anzawa R, Kashiwagi Y, Ito K, Katoh D, Fujisaki M, Kayama Y, Date Taro, Hongo K, Yoshimura M.
  • Journal Title: J Endocrinol Volume: (in press)
  • DOI: 10.1530/JOE-14-0067
  • Peer Reviewed
• [Journal Article] A technique for quantifying intracellular free sodium ion using a microplate reader in combination with sodium-binding benzofuran isophthalate and probenecid in cultured neonatal rat cardiomyocytes (2013)
  • Author(s): Katoh D, Hongo K, Ito K, Yoshino T, Kayama Y, Komukai K, Kawai M, Date T, Yoshimura M.
  • Journal Title: BMC Res Notes Volume: 6 Pages: 556
  • DOI: 10.1186/1756-0500-6-556
  • Peer Reviewed
• [Journal Article] Rapid induction of aldosterone synthesis in cultured neonatal rat cardiomyocytes under high glucose conditions (2013)
  • Author(s): Fujisaki M, Nagoshi T, Nishikawa T, Date T, Yoshimura M.
  • Journal Title: BioMed Res Inter. Volume: 2013 Pages: 161396
  • DOI: 10.1155/2013/161396
  • Peer Reviewed
• [Journal Article] Transient decrease in serum potassium level during ischemic attack of acute coronary syndrome : Paradoxical contribution of plasma glucose level and glycohemoglobin (2013)
  • Author(s): Sekiyama H, Nagoshi T, Komukai K, Matsushima M, Katoh D, Ogawa K, Minai K, Ogawa T, Yoshimura M.
  • Journal Title: Cardiovasc Diabetol Volume: 12 Pages: 4
  • DOI: 10.1186/1475-2840-12-4
  • Peer Reviewed
• [Journal Article] Biphasic action of aldosterone on Akt signaling in cardiomyocytes (2012)
  • Author(s): Nagoshi T, Date T, Fujisaki M, Yoshino T, Sekiyama H, Ogawa K, Kayama Y, Minai K, Komukai K, Ogawa T, Yoshimura M.
  • Journal Title: Horm Metab Res. Volume: 44(13) Pages: 931-7
  • DOI: 10.1055/s-0032-1316343
  • Peer Reviewed
• [Journal Article] The role of Na+/H+ exchanger in Ca2+ overload and ischemic myocardial damage in hearts from type 2 diabetic db/db mice (2012)
  • Author(s): Anzawa R, Seki S, Nagoshi T, Taniguchi I, Feuvray D, Yoshimura M.
  • Journal Title: Cardiovasc Diabetol Volume: 11 Pages: 33
  • DOI: 10.1186/1475-2840-11-33
  • Peer Reviewed
• [Journal Article] Optimization of cardiac metabolism in heart failure (2011)
  • Author(s): Nagoshi T, Yoshimura M, Rosano GM, Lopaschuk GD, Mochizuki S.
  • Journal Title: Curr Pharm Des. Volume: 17(35) Pages: 3846-53
  • DOI: 10.2174/138161211798357773
  • Peer Reviewed
• [Presentation] Aldosterone Preconditioning is Protective during Ischemia-Reperfusion through Modulation of p38 MAPK Signaling in Isolated Rat Heart (2014)
  • Author(s): 吉野拓哉、吉村道博、他
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京都千代田区東京国際フォーラム
  • Year and Date: 2014-03-22
• [Presentation] アルドステロンの合成と作用に関する新しい知見 (2014)
  • Author(s): 吉村道博
  • Organizer: 第43回日本心脈管作動物質学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2014-02-16
  • Invited
• [Presentation] Rapid Induction of Aldosterone Cascade Exerts Cardioprotective Effect During ex vivo Ischemia-reperfusion Injury in a Mineralocorticoid Receptor-independent Manner (2013)
  • Author(s): 吉野拓哉、吉村道博、他
  • Organizer: アメリカ心臓病学会学術集会
  • Place of Presentation: アメリカ合衆国テキサス州ダラス
  • Year and Date: 2013-11-19
• [Presentation] 食塩と循環器疾患に関する最新の話題 (2013)
  • Author(s): 吉村道博
  • Organizer: 第77回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-17
• [Presentation] Significance of Monitoring Potassium Level in Parallel with Glucose Level in Acute Coronary Syndorome (2013)
  • Author(s): 吉村道博
  • Organizer: 第77回日本循環器学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-16