2013 Fiscal Year Final Research Report
Preventive effects of 5-HT agonists on the abnormal development of of 5-HTergic system induced by prenatal ethanol exposure in rats
| Project/Area Number |
23591595
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | The University of Tokushima |
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Principal Investigator |
FUKUI Yoshihiro 徳島大学, ヘルスバイオサイエンス研究部, 教授 (50144168)
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| Co-Investigator(Kenkyū-buntansha) |
SAKATA Hiromi 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (50294666)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 先天異常 / セロトニン / 5-HT1A受容体作動薬 / 5-HT２A/2C受容体作動薬 / 脳発達 / エタノール |
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| Research Abstract |
We administered the 5-HT1A agonist, ipsapirone, or the 5-HT2A/2C agonist, DOI, against prenatal ethanol (EtOH)-exposed rats during E13-20. Based on our immunohistochamical observation on E20, administrations of both agonists prevented a reduction in the number of 5-HTergic neurons in the midbrain raphe nuclei induced by EtOH exposure during E10-20. Ipsapirone also alleviated an abnormality of anxiety-like behavior seen in an elevated-plus maze test caused by prenatal EtOH exposure. The quantitative analysis of gene expression in fetal brains revealed that an expression of Phox2b, which is related to development of 5-HT neurons, was reduced after prenatal exposure to EtOH, but DOI attenuated the effect of EtOH on Phox2b gene expression. We concluded that the effect of prenatal EtOH exposure on development of 5-HT neurons and behavioral changes resulting in the altered 5-HTergic systems could be alleviated by the enhancement of signaling via 5-HT1A or 5-HT2A/2C receptors during E13-20.
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