2013 Fiscal Year Final Research Report
Molecular basis of melanomagenesis based on the failure of pluripotency maintenance of melanocyte stem cells
| Project/Area Number |
23591612
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Akita University |
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Principal Investigator |
MANABE Motomu 秋田大学, 医学(系)研究科(研究院), 教授 (30138309)
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| Project Period (FY) |
2011 – 2013
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| Keywords | メラノーマ / スフェロイド / 三酸化ヒ素 / サリノマイシン |
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| Research Abstract |
Recurrence after chemotherapy is a major cause of cancer mortality: subsets of tumor cells evade initial chemotherapy or radiotherapy and survive to re-propagate the tumor. To develop a novel therapeutic approach for melanoma, we applied a non-adhesive culture system which developed spheroids mimicking the properties of melanoma in vivo. Subsequently, spheroids involved cells exhibiting clonogenic and slow-cycling properties in addition to chemotherapeutic resistance to doxorubicin. Interestingly, while treatment of spheroids with either salinomycin or As2O3 showed limiting effects, a combinatorial treatment was markedly superior to single treatment with each drug. Thus, melanoma spheroids could be a new platform for studying melanoma biology and are likely to provide a clinically relevant target for the novel chemotherapy.
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