An establishment of new tumor dormancy therapy based on regulation of radical scavenger

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591967
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Oita University
• Principal Investigator: INOMATA MASAFUMI 大分大学, 医学部, 准教授 (60315330)
• Co-Investigator(Kenkyū-buntansha): KITANO Seigou 大分大学, 法人本部, 学長 (90169871) ; MORIYAMA Masatsugu 大分大学, 医学部, 教授 (90239707) ; NOGUCHI Takayuki 大分大学, 医学部, 病院長 (90156183) ; ETOH Tsuyoshi 大分大学, 医学部, 講師 (00404369) ; HIRATSUKA Takahiro 大分大学, 医学部, 医員 (20600886)
• Project Period (FY): 2011 – 2013
• Keywords: 小腸大腸肛門外科学 / 腫瘍学

Research Abstract

We investigated whether new antioxidants, alfa-lipoic acid (ALA) delivertives and vitamin E delivertives elicits its antiproliferative effects in vivo and in vitro by inducing apoptosis, autophagy or cell cycle arrest, and we analyzed the expression of proteins related to these phenomena and their phosphorylation in HT-29 human colon cancer cells. These new antioxidants inhibited the proliferation of HT-29 cells through the mechanisms of G2/M cell cycle arrest and autophagy but not that of apoptosis. The newly synthesized ALA derivative may be expected to become a novel cancer therapeutic strategy through its induction of autophagy.

Research Products

• [Journal Article] Bolus injection of newly synthesized vitamin E derivative ETS-GS for the treatment of acute severe ulcerative colitis in a mouse model. New vitamin E derivative for acute severe UC. (2013)
  • Author(s): Hiratsuka T, Inomata M, Hagiwara S, Kono Y, Shiraishi N, Noguchi T, Kitano S.
  • Journal Title: Int J colorectal Dis. Volume: 28(3) Pages: 305-11
  • DOI: 10.1007/s00384-012-1502–y
  • Peer Reviewed
• [Journal Article] DHL-TauZnNa, a newly synthesizedα-lipoic acid derivative, induces autophagy in human colorectal cancer cells (2013)
  • Author(s): Hiratsuka T, Inomata M, Kono Y, Yokoyama S, Shiraishi N, Kitano S.
  • Journal Title: Oncol Rep. Volume: 29(6) Pages: 2140-2146
  • DOI: 10.3892/or.2013.2394
  • Peer Reviewed
• [Journal Article] A newly synthetic vitamin E derivative, E-Ant-S-GS, attenuates lung injury caused by cecal ligation and puncrure- unduced sepsis in rats (2012)
  • Author(s): Kono Y, Inomata M, Hagiwara S, Shiraishi N, Noguchi T, Kitano S.
  • Journal Title: Surgery Volume: 151(3) Pages: 420-426
  • DOI: 10.1016/j.surg.2011.08.003
  • Peer Reviewed
• [Journal Article] Antiproliferative effects of a newα-lipoic acid derivative, DHL-HisZnNa, in HT29 human colon cancer cells in vitro (2012)
  • Author(s): Kono Y, Inomata M, Hagiwara S, Hiratsuka T, Suzuki K, Koga H, Shiraishi N, Noguchi T, Kitano S.
  • Journal Title: Expert Opinion On Therapeutic Targets Volume: 16 Pages: S103-109
  • DOI: 10.1517/14728222.2011.640320
  • Peer Reviewed
• [Presentation] 新規αリポ酸誘導体DHLHZnのオキサプラチン誘導末梢神経障害に対する抑制効果;ラットモデルにおける検討 (2012)
  • Author(s): 杉田諭、二日市琢良、草野徹、中嶋健太郎、平塚孝宏、赤木智徳、柴田智隆、上田貴威、當寺ヶ盛学、白下英史、衛藤剛、猪股雅史、野口剛、白石憲男、北野正剛
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2012-12-03
• [Presentation] Antitumor effects of a newα-lipoic acid derivative,DHL-HisZnNa,are mediated by G1 cell cycle arrest in HT29 human colon cancer cells (2011)
  • Author(s): Yohei Kono, Masafumi Inomata, Takahiro Hiratsuka, Takashi Masuda, Teijiro Hirashita, Fumitaka Yoshizumi, Norio shiraishi, Seigo Kitano
  • Organizer: International Surgical Week 2011
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-08-29
• [Presentation] The alpha-lipoic acid derivative, sodium zinc dihydrolipoylhistidinate (DHLHZn), reduces chemotherapy- induced alopecia in a rat model (2011)
  • Author(s): Masafumi Inomata, Yohei kono, Takahiro Hiratsuka, Fumitaka Yoshizumi, Norio Shiraishi, Satoshi Hagiwara, Tsuyoshi Noguchi, Seigo Kitano
  • Organizer: International Surgical Week 2011
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2011-08-28
• [Presentation] Effect of A Newα-lipoic Acid Dervative, DHL-HisZn, on Cancer Cell Growth in HT29 Human Colon Cancer Cell (2011)
  • Author(s): Yohei Kono, Masafumi Inomata, Takahiro Hiratsuka, Kazumi Ogata, Seigo Kitano
  • Organizer: OOTR 7th Annual Conference
  • Place of Presentation: 香港
  • Year and Date: 2011-05-13
• [Presentation] The Effectiveness of A New Alpha-lipoic Acid Derivative, Sodium Zinc Dihydrolipoylhistidenate (DHLHZn), in Preventing Chemotherapy-induced Alopecia (2011)
  • Author(s): Masafumi Inomata, Yohei Kono, Takahiro Hiratsuka, Kazumi Ogata, Seigo Kitano
  • Organizer: OOTR 7th Annual Conference
  • Place of Presentation: 香港
  • Year and Date: 2011-05-13
• [Presentation] Antiproliferative Effect of Newly Synthetic Antioxidant,αLipoic Acid Derivatives (2011)
  • Author(s): Takahiro Hiratsuka, Masafumi Inomata, Yohei Kono, Kazumi Ogata, Seigo Kitano
  • Organizer: OOTR 7th Annual Conference
  • Place of Presentation: 香港
  • Year and Date: 2011-05-13