2013 Fiscal Year Final Research Report
Sp1 interference prevents joint destruction of RA through inhibitory effects of gliostatin
| Project/Area Number |
23592225
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
NAGAYA Yuko 名古屋市立大学, 医学(系)研究科(研究院), 教授 (90291583)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUKA Takanobu 名古屋市立大学, 大学院医学研究科, 教授 (10185316)
ASAI Kiyofumi 名古屋市立大学, 大学院医学研究科, 教授 (70212462)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 関節リウマチ / 滑膜細胞 / グリオスタチン / Sp1 |
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| Research Abstract |
Gliostatin/thymidine phosphorylase (GLS/TP) has angiogenic and arthritogenic activities, and aberrant GLS production has been observed in the active synovial membranes of rheumatoid arthritis (RA) patients. The human GLS gene promoter contains at least seven consensus binding sites for the DNA binding protein Sp1. Here we examined whether Sp1 is necessary for GLS production in RA.Mithramycin is an aureolic-acid anti-neoplastic antibiotic used for treating cancer-related hypercalcaemia, leukemia and testicular cancer, which prevents Sp1 binding to its cognate site in DNA by modifying CG sequences. We also studied the effects of the Sp1 inhibitor mithramycin on GLS production in RA fibroblast-like synoviocytes.
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[Journal Article] Cigarette smoke condensate extracts induce IL-1 beta production from rheumatoid arthritis patient-derived synoviocytes, but not osteoarthritis patient-derived synoviocytes, through aryl hydrocarbon receptor-dependent NF-kappa B activation and novel NF-kappa B sites2013
Author(s)
Adachi M, Okamoto S, Chujyo S, Arakawa T, Yokoyama M, Yamada K, Hayashi A, Akita K, Takeno M, Ito S, Takii T, Waguri-Nagaya Y, Otsuka T, Hayakawa K, Miyazawa K, Onozaki K
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Journal Title
J Interferon & Cytokine Research
Volume: 33
Pages: 297-307
DOI
Peer Reviewed
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[Presentation] Gliostatin regulates vascular endothelial growth-factor production in human fibroblast-like synoviocytes2014
Author(s)
Kawaguchi Y, Waguri-Nagaya Y, Tatematsu N, Kobayashi M, Goto H, Nozaki M, Ikuta K, Aoyama M, Asai K, Otsuka T
Organizer
Annual European Congress of Rheumatology
Place of Presentation
Le Palais des Congres de Paris, France (発表確定)
Year and Date
20140611-14
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