Elucidation of the cancer stem cell activation mechanism by EMT in invasion and metastasis of head and neck cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592537
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nara Medical University
• Principal Investigator: OTA Ichiro 奈良県立医科大学, 医学部, 助教 (00326323)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Akihisa 群馬大学, 先端科学研究指導者育成ユニット, 准教授 (60275336)
• Project Period (FY): 2011 – 2013
• Keywords: 頭頸部がん / 癌幹細胞 / 浸潤・転移 / EMT

Research Abstract

In head and neck cancer cells, we investigated whether Wnt/Snail signaling pathway could induce epithelial-mesenchymal transition, EMT, promote the activation of cancer stem cells, and enhance cancer cel invasion and metastasis. As a result, it was found in the in vivo and in vitro study that Wnt/Snail signaling pathway could induce cancer invasion and metastasis though EMT. In addition, the cancer cells induced EMT by Snail could acquire the cancer stem cell-like properties, such as enhancement of treatment-resistant and expression of cancer stem cell marker. Controlling these signaling pathways involved in EMT will lead to controlling the invasion and metastasis of head and neck cancer.

Research Products

• [Journal Article] Snail-induced epithelial-mesenchymal transition promotes cancer stem cell-like phenotype in head and neck cancer cells (2014)
  • Author(s): Masui T, Ota I, Yook JI, Mikami S, Yane K, Yamanaka T, Hosoi H
  • Journal Title: Int J Oncol Volume: 44 Pages: 693-699
  • Peer Reviewed
• [Journal Article] Sentinel Lymph Node Detection in Patients with Oral Cancer by MR Lymphography using Superparamagnetic Iron Oxide (2013)
  • Author(s): Uemura H, Ota I, Fujii T, Suzuki M, Sakai M, Nakanishi K, Tomita Y, Noguchi A, Hosoi H, Yoshino K
  • Journal Title: The Open Otorhinolaryngology Journal Volume: 7 Pages: 14-18
  • Peer Reviewed
• [Journal Article] Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells (2013)
  • Author(s): Ota I, Higashiyama S, Masui T, Yane K, Hosoi H, Matsuura N
  • Journal Title: Oncol Rep Volume: 30 Pages: 1593-1600
  • Peer Reviewed
• [Journal Article] Expression of REG III and prognosis in head and neck cancer (2013)
  • Author(s): Masui T, Ota I, Itaya-Hironaka A, Takeda M, Kasai T, Yamauchi A, Sakuramoto- Tsuchida S, Mikami S, Yane K, Takasawa S, Hosoi H
  • Journal Title: Oncol Rep Volume: 30 Pages: 573-578
  • Peer Reviewed
• [Journal Article] Depression of p53- independent Akt survival signals in human oral cancer cells bearing mutated p53 gene after exposure to high-LET radiation (2012)
  • Author(s): Nakagawa Y, Takahashi A, Kajihara A, Yamakawa N, Imai Y, Ota I, Okamoto N, Mori E, Noda T, Furusawa Y, Kirita T, Ohnishi T
  • Journal Title: Biochem Biophys Res Commun Volume: 423(4) Pages: 654-60
  • Peer Reviewed
• [Journal Article] Nuclear hormone receptor corepressor promotes esophageal cancer cell invasion by transcriptional repression of interferon-γ-inducible protein 10 in a casein kinase 2-dependent manner (2012)
  • Author(s): Yoo JY, Choi HK, Choi KC, Park SY, Ota I, Yook JI, Lee YH, Kim K, Yoon H
  • Journal Title: Mol Biol Cell Volume: 23(15) Pages: 2943-2954
  • Peer Reviewed
• [Journal Article] Therapeutic strategies for head and neck cancer based on p53 status (2012)
  • Author(s): Ota I, Okamoto N, Yane K, Takahashi A, Masui T, Hosoi H, Ohnishi T
  • Journal Title: Experimental and Therapeutic Medicine Volume: 3 Pages: 585-591
  • Peer Reviewed
• [Journal Article] siRNA targeted for NBS1 enhances heat sensitivity in human anaplastic thyroid carcinoma cells (2011)
  • Author(s): Okamoto N, Takahashi A, Ota I, Ohnishi K, Mori E, Kondo N, Noda T, Nakagawa Y, Uemura H, Yane K, Hosoi H, Ohnishi T
  • Journal Title: Int J Hyperthermia Volume: 27 Pages: 297-304
  • Peer Reviewed
• [Presentation] Low-intensity ultrasound enhances the anti-cancer activity of cetuximab in human head and neck cancer cells (2013)
  • Author(s): Ota I, Masui T, Uemura K, Kanno M, Yane K, Hosoi H
  • Organizer: III Eurasian Head & Neck Cancer Forum
  • Place of Presentation: Petersburg, Russia
  • Year and Date: 20130725-28
• [Presentation] 糖尿病と癌の進展-高血糖は癌の浸潤を促進させる(1)- (2013)
  • Author(s): 太田一郎、桝井貴史、三上慎司、細井裕司
  • Organizer: 日耳鼻大阪地方連合会327回例会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-12-07
• [Presentation] 下咽頭癌におけるREGIIIの発現と治療感受性 (2013)
  • Author(s): 桝井貴史、太田一郎、広中安佐子、武田麻衣子、笠井孝彦、山内晶世、土田澄代、高沢伸、細井裕司
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 東京都
  • Year and Date: 2013-06-04
• [Presentation] がん研究UP TO DATE 2013世界の基礎研究の動向 (2013)
  • Author(s): 太田一郎
  • Organizer: 第7回頭頸部癌基礎研究会
  • Place of Presentation: 東京都
  • Year and Date: 2013-06-03
• [Presentation] 糖尿病と癌の進展-高血糖は癌の浸潤を促進させる- (2013)
  • Author(s): 太田一郎、桝井貴史、三上慎司、細井裕司
  • Organizer: 日本耳鼻咽喉科学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2013-05-18
• [Presentation] Snail1 regulates MMP- dependent invasive activities through cancer cell EMT programs in head and neck cancer cells (2012)
  • Author(s): Ota I, Masui T, Yane K, Okamoto N, Hosoi H
  • Organizer: 8th International Conference on Head & Neck Cancer
  • Place of Presentation: the Metro Toronto Convention , Centre in Toronto, Ontario, Canada
  • Year and Date: 20120721-25
• [Presentation] Snail1 regulates MMP- dependent invasive activities through cancer cell EMT programs in head and neck cancer cells (2012)
  • Author(s): Ota I, Masui T, Yane K, Okamoto N, Hosoi H
  • Organizer: the 14th Japan-Korea Joint Meeting of Otorhinolaryngology- Head and Neck Surgery
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20120412-14
• [Presentation] Low-intensity ultrasound enhances anti-cancer effect of cetuximab on apoptosis induction in human head and neck cancer cells (2012)
  • Author(s): Masui T, Ota I, Kanno M, Hosoi H
  • Organizer: the 14th Japan-Korea Joint Meeting of Otorhinolaryngology- Head and Neck Surgery
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20120412-14
• [Presentation] 頭頸部癌細胞におけるEGFRをターゲットにした分子標的治療の超音波照射による増感効果 (2012)
  • Author(s): 太田一郎, 桝井貴史, 神野正敏, 家根旦有, 細井裕司
  • Organizer: 第18回国際癌増感研究会
  • Place of Presentation: 吹田市
  • Year and Date: 2012-06-09
• [Presentation] 超音波照射によるEGFRをターゲットにした分子標的治療Cetuximabの増感効果の検討 (2012)
  • Author(s): 太田一郎, 桝井貴史, 神野正敏, 家根旦有, 細井裕司
  • Organizer: 第36回日本頭頸部癌学会
  • Place of Presentation: 松江市
  • Year and Date: 2012-06-07
• [Presentation] 下咽頭癌におけるREG遺伝子ファミリーの発現と予後 (2012)
  • Author(s): 桝井貴史、太田一郎、高沢伸、細井裕司
  • Organizer: 第36回日本頭頸部癌学会
  • Place of Presentation: 松江市
  • Year and Date: 2012-06-07
• [Presentation] がん研究UP TO DATE 2012世界の基礎研究の動向 (2012)
  • Author(s): 太田一郎
  • Organizer: 第6回頭頸部癌基礎研究会
  • Place of Presentation: 松江市
  • Year and Date: 2012-06-06
• [Presentation] Combination of cetuximab and low-intensity ultrasound causes a synergistic enhancement in cell killing and an additive enhancement in apoptosis induction in human head and neck cancer cells (2011)
  • Author(s): Masui T, Ota I, Kanno M, Hosoi H
  • Organizer: The 11th Japan- Taiwan Conference on Otolaryngology- Head and Neck Surgery
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2011-12-08
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~oto/index.html