2013 Fiscal Year Final Research Report
Identification of stem cell marker of mouse corneal epithelial cell
| Project/Area Number |
23592607
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
SHIRAISHI Atsushi 愛媛大学, 医学(系)研究科(研究院), 准教授 (90314963)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Takeshi 愛媛大学, 大学院医学系研究科, 寄附講座助教 (70380285)
SHIRAKATA Yuji 愛媛大学, 大学院医学系研究科, 准教授 (50226320)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 再生医療 / 角膜上皮 / 形質転換 |
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| Research Abstract |
We have established the mouse model for trans-differentiation from epidermal cell to corneal epithelial cell using K12-Cre-Zeg mouse in which green fluorescein protein (GFP) is expressed driven by Keratin 12 promoter. We first examined immunohistochemical staining, and results showed that K 10 was expressed in the center of the cornea and K10 and K12 were expressed in the limbus of the cornea in the trans-differentiation model. When side population cells (Sp cells) isolated from epidermis were co-cultured with cornel keratocyte isolated from corneal limbus, GFP positive cells were detected. RT-PCR results also showed positive for K 12. We then employed transcriptome analysis between the keratocytes isolated from central and limabal cornea. The results showed high expression of Wnt signal related mRNA were detected.
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[Journal Article] Association of Toll-like receptor 4 gene polymorphisms in Japanese subjects with primary open-angle, normal-tension, and exfoliation glaucoma2012
Author(s)
Takano Y, Shi D, Shimizu A, Funayama T, Mashima Y, Yasuda N, Fukuchi T, Abe H, Ideta H, Zheng X, Shiraishi A, Ohashi Y, Nishida K, Nakazawa T, Fuse N
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Journal Title
Am J Ophthalmol
Volume: 154(5)
Pages: 825-832.e1
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[Journal Article] Assessment of real-time polymerase chain reaction detection of Acanthamoeba and prognosis determinants of Acanthamoeba keratitis2012
Author(s)
Ikeda Y, Miyazaki D, Yakura K, Kawaguchi A, Ishikura R, Inoue Y, Mito T, Shiraishi A, Ohashi Y, Higaki S, Itahashi M, Fukuda M, Shimomura Y, Yagita K
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Journal Title
Ophthalmology
Volume: 119(6)
Pages: 1111-9
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[Journal Article] Mathematical projection model of visual loss due to fuchs corneal dystrophy2011
Author(s)
Hatou S, Shimmura S, Shimazaki J, Usui T, Amano S, Yokogawa H, Kobayashi A, Zheng X, Shiraishi A, Ohashi Y, Inatomi T, Tsubota K
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 52(11)
Pages: 7888-93
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