2013 Fiscal Year Final Research Report
A basic research to develop a new therapy for the treatment of diabetic ulcer using skin derived precursor cells
| Project/Area Number |
23592645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
EBISAWA Katsumi 名古屋大学, 医学部附属病院, 病院助教 (20397459)
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| Co-Investigator(Kenkyū-buntansha) |
KAMEI Yuxuru 名古屋大学, 医学(系)研究科, 教授 (10257678)
TORIYAMA Kazuhiro 名古屋大学, 医学(系)研究科, 准教授 (40314017)
YAGI Shinjirou 名古屋大学, 医学部附属病院, 講師 (00378192)
SATOU Hideyoshi 名古屋大学, 医学部附属病院, 医員 (70528968)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 皮膚由来前駆細胞 / 糖尿病性潰瘍 / 創傷治癒 |
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| Research Abstract |
We investigate the influence of SKPs on diabetic wound healing. Skins were harvested from 4 week-old male diabetic mice (db/db), and were minced and digested to isolate SKPs. Then SKPs were cultured for three weeks and labeled with PKH 26 before use. We used the excisional wound splint model on diabetic mice. The wounds were randomly divided into control group and SKPs group.We took photographs of the experimental area and harvested tissue samples, and evaluated time to wound closure and histological findings. Wounds treated with SKPs demonstrated a significantly decreased time to closure compared with control wounds. Histological analysis showed that the Capillary Score was significantly higher in SKPs-treated wounds at day 10 but not at day 28. Nerve Density had increased significantly in SKPs-treated wounds at day 28 compared with control group. Some applied SKPs were stained by NF-H, which demonstrates that SKPs directly differentiated into neurons.
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