2013 Fiscal Year Final Research Report

Comprehensive analysis of intracellular proteins on the cell surface for elucidation of their roles in tissue damage and repair.

Research Project

Project/Area Number	23592670
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Emergency medicine
Research Institution	Yamaguchi University
Principal Investigator	IZUMI Tomonori 山口大学, 医学(系)研究科(研究院), 准教授 (00261694)
Project Period (FY)	2011 – 2013
Keywords	機能プロテオーム解析 / 細胞表面標識 / 細胞質漏出 / 炎症メディエーター / 疾患マーカー
Research Abstract	Although various intracellular proteins are released from damaged tissues, functional impact of these proteins in the extracellular space remains largely unknown. To characterize intracellular proteins potentially functioning in the extracellular space and affecting cellular responses, cell surface-associated proteins were selectively identified using a combination of cell surface labeling and mass spectrometry-based protein identification technology. We identified 454 proteins, including 405 putative intracellular proteins, on the surface of U937 cells. Besides a variety of high-abundance proteins, the protein subset included some of clinical markers, such as HMGB1. Out of the 405 proteins, 162 proteins were also found to be released from damaged HEK293 cells. Furthermore, a certain protein was confirmed to affect a signal transduction pathway in U937 cells.

(3 results)