2013 Fiscal Year Final Research Report
SNP typing of the cariogenic protein gene
| Project/Area Number |
23592790
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHTA Toru 北海道医療大学, 個体差健康科学研究所, 教授 (10223885)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 遺伝子多型 |
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| Research Abstract |
To evaluate the discrimination method of HLA Alleles, we developed the detection method by PCR-Sequence Specific Primer (SSP) and the melt peak analysis (Tm). The frequencies of target Allele calculated from database were less than 10%, therefore our method could discriminate more than 90% samples.
We analyzed the Buffer capacity of saliva and Carbonic Anhydrase VI SNP of Children attended permanent caries preventing program. There were a positive association between caries present and buffer capacity. We could distinguish the rs2274327 (C/T) polymorphism by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
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