Development of tissue engineered oral mucosa maintaining higher regenerative potential -application of oral mucosa progenitor/stem cells-

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592982
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: YOSHIZAWA Michiko 新潟大学, 医歯学総合病院, 講師 (60303137)
• Co-Investigator(Kenkyū-buntansha): KENJI Izumi 新潟大学, 医歯学系, 教授 (80242436)
• Co-Investigator(Renkei-kenkyūsha): TERASHI Hiroto 神戸大学, 医学部, 教授 (80217421)
• Project Period (FY): 2011 – 2013
• Keywords: 移植・再生医療 / 口腔顎顔面再建外科学 / 口腔粘膜

Research Abstract

We have investigated the application of ex vivo-produced oral mucosa equivalent (EVPOME) and its ability of oral mucosa regeneration. However, cultivated epithelial cells using the present methods has heterogeneity, consequently, there is a risk that EVPOME possess poor cell growth activity and capability of oral mucosa regeneration. The objective of the present study was to investigate the capability of EVPOME fabricated with small-sized cell population in which oral mucosal progenitor/stem-cell-enriched subpopulation present and the change after EVPOME grafting subcutaneously in mice histologically. More Ki-67 positive cells were observed in the epithelial cells of EPOME fabricated by small-sized cell population. Epithelial elongation of EVPOME fabricated by small-sized cell population occurs faster than that of EVPOME using present methods. These findings suggest that EVPOME with small-sized cell population has high activity and capability of oral mucosal regeneration.

Research Products

• [Journal Article] Histochemical aspects of the vascular invasion at the erosion zone of the epiphyseal cartilage in MMP-9-deficient mice (2013)
  • Author(s): Kojima T, Hasegawa T, DE Freitas PH, Yamamoto T, Sasaki M, Horiuchi K, Hongo H, Yamada T, Sakagami N, Saito N, Yoshizawa M, Kobayashi T, Maeda T, Saito C, Amizuka N
  • Journal Title: Biomed Res Volume: 34(3) Pages: 119-28
  • Peer Reviewed
• [Journal Article] Regeneration Process after Intraoral Grafting of Tissue-Engineered Human Oral Mucosa in Athymic Mice (2011)
  • Author(s): Yoshizawa M, Koyama T, Funayama A, Mikami T, Saito C
  • Journal Title: Oral Craniofacial Engineering Volume: 1(4) Pages: 376
  • Peer Reviewed
• [Presentation] 口腔粘膜上皮前駆/幹細胞を応用した培養複合口腔粘膜の皮下移植後の動態 (2014)
  • Author(s): 芳澤享子、小山貴寛、船山昭典、三上俊彦、小野由起子、小林正治
  • Organizer: 第68回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20140507-09
• [Presentation] Remodeling of the basement membrane during the healing process after intraoral grafting of tissue-enginreed human oral mucosa in athymic mice (2013)
  • Author(s): Yoshizawa M, Koyama T, Funayama A, Mikami T, Kojima T, Niimi K, Kobayashi T, Saito C
  • Organizer: 21th International Conference on oral and maxillofacial surgery
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20131021-24
• [Presentation] 培養複合口腔粘膜の皮下移植モデルにおける培養上皮の動態 (2013)
  • Author(s): 芳澤享子,小山貴寛,船山昭典,三上俊彦,小野由起子,齊藤力
  • Organizer: 第67回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 広島
  • Year and Date: 20130523-24
• [Presentation] 培養複合口腔粘膜移植後の口腔粘膜再生過程における基底膜の動態 (2012)
  • Author(s): 芳澤享子、小山貴寛、船山昭典、三上俊彦、小野由起子、齊藤力
  • Organizer: 第66回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 宇都宮
  • Year and Date: 20120517-18
• [Remarks]
  • URL: http://www.dent.niigata-u.ac.jp/surgery1/classroom02.html
• [Remarks]
  • URL: http://bmrctr.jp/saisei/project/project3