The effect of IL-22 on biological activity of periodontal ligament cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23593076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Hyogo Medical University
• Principal Investigator: OHYAMA hideki 兵庫医科大学, 医学部, 准教授 (90280685)
• Co-Investigator(Kenkyū-buntansha): TERADA Nobuyuki 兵庫医科大学, 医学部, 名誉教授 (50150339) ; NAKASHO Keiji 兵庫医科大学, 医学部, 教授 (00217712) ; YAMADA Naoko 兵庫医科大学, 医学部, 講師 (10319858) ; YAMANEGI Koji 兵庫医科大学, 医学部, 講師 (00434944)
• Project Period (FY): 2011 – 2013
• Keywords: 歯根膜細胞 / IL-22 / 石灰化誘導

Research Abstract

This study focused on the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. We found that IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. In addition, the production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner.IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.

Research Products

• [Journal Article] Decreased expression of VE-cadherin and claudin-5 and increased phosphorylation of VE-cadherin in vascular endothelium in nasal polyps (2013)
  • Author(s): Yukitatsu Y, Hata M, Yamanegi K, Yamada N, Ohyama H, Nakasho K, Kojima Y, Oka H, Tsuzuki K, Sakagami M, Terada N
  • Journal Title: Cell Tissue Res Volume: Jun;352(3) Pages: 647-57
  • DOI: 10.1007/s00441-013-1583-0
  • Peer Reviewed
• [Journal Article] T-cell Responses Involved in the Predisposition to Periodontal Disease : Lessons from Immunogenetic Studies of Leprosy (2012)
  • Author(s): Ohyama H, Kato-Kogoe N, Takeuchi-Hatanaka K, Yamanegi K, Yamada N, Nakasho K, Matsushita S, Terada N
  • Journal Title: J Clin Cell Immunol Volume: S1 Pages: 005
  • DOI: 10.4172/2155-9899.S1-005
  • Peer Reviewed
• [Journal Article] The promotional effect of IL-22 on mineralization activity of periodontal ligament cells (2012)
  • Author(s): Kato-Kogoe N, Nishioka T, Kawabe M, Kataoka F, Yamanegi K, Yamada N, Hata M, Yamamoto T, Nakasho K, Urade M, Terada N, Ohyama H
  • Journal Title: Cytokine Volume: Jul;59(1) Pages: 41-8
  • DOI: 10.1016/j.cyto.2012.03.024
  • Peer Reviewed
• [Presentation] IL-22/TNF-αの単独および共刺激によって誘導されるヒト歯根膜由来細胞の生物学的活性 (2013)
  • Author(s): 川辺睦記,浦出雅裕,大山秀樹
  • Organizer: 第67回日本口腔科学会学術集会
  • Place of Presentation: 栃木県総合文化センター(栃木県)
  • Year and Date: 2013-05-23
• [Presentation] Th17細胞産生性サイトカインが血管石灰化に及ぼす影響 (2012)
  • Author(s): 大山秀樹
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2012-04-27
• [Presentation] IL-22がヒト歯根膜由来細胞の石灰化ノジュール形成に及ぼす影響 (2011)
  • Author(s): 大山秀樹
  • Organizer: 第54回日本歯周病学会春季学術大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-05-28
• [Presentation] IL-22存在下においてヒト歯根膜由来細胞が発現する遺伝子の網羅的解析 (2011)
  • Author(s): 川辺睦記
  • Organizer: 第54回日本歯周病学会春季学術大会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-05-27
• [Book] Leprosy-Science working towards dignity (2011)
  • Author(s): Hideki Ohyama
  • Total Pages: 97-107
  • Publisher: Tokai University Press