Rapid screening of gene(s) related to the phenotype of model rats with high levels of voluntarily wheel running activity

2011 Fiscal Year Final Research Report

• Project/Area Number: 23650406
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: The University of Tokushima
• Principal Investigator: IMOTO Issei (TACHIBANA Issei) 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (30258610)
• Co-Investigator(Kenkyū-buntansha): NAKAYA Yutaka 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (50136222) ; NIKAWA Takeshi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (20263824)
• Co-Investigator(Renkei-kenkyūsha): TAJIMA Atsushi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (10396864)
• Project Period (FY): 2011
• Keywords: 高運動習性ラット / ゲノム / 次世代シーケンサー / 連鎖解析

Research Abstract

In order to identify gene(s) and molecular pathway(s) possibly related to the phenotype of model rats with high levels of voluntarily wheel running activity(Spontaneously-Running-Tokushima-Shikoku, SPORTS) established from Wistar rats, we established a novel integrated screening system through combining exome sequencing using a next-generation sequencer, linkage analysis, and selection algorithm using genome and polymorphism databases. Through this study, we successfully established a novel screening system for rat genome analysis and identified a set of candidate genes related to the phenotype of SPORTS rats.

Research Products

• [Journal Article] The incidence of hypoplasia of the corpus callosum in patients with dup(X)(q28) involving MECP2 is associated with the location of distal breakpoints (2012)
  • Author(s): Honda S, Hayashi S, Nakane T, Imoto I, Kurosawa K, Mizuno S, Okamoto N, Kato M, Yoshihashi H, Kubota T, Nakagawa E, Goto YI, Inazawa J
  • Journal Title: Am J Med Genet A Volume: (in press)
  • DOI: doi:10.1002/ajmg.a.35321
  • Peer Reviewed
• [Journal Article] SIX1 promotes epithelialmesenchymal transition in colorectal cancer through ZEB1 activation (2012)
  • Author(s): Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J
  • Journal Title: Oncogene Volume: (in press)
  • DOI: doi:10.1038/onc.2011.646.
  • Peer Reviewed
• [Journal Article] Deletion at chromosome 10p11. 23-p12. 1 defines characteristic phenotypes with marked midface retrusion (2012)
  • Author(s): Okamoto N, Hayashi S, Masui A, Kosaki R, Oguri I, Hasegawa T, Imoto I, Makita Y, Hata A, Moriyama K, Inazawa J
  • Journal Title: J Hum Genet Volume: 57(3) Pages: 191-6
  • DOI: doi:10.1038/jhg.2011.154
  • Peer Reviewed
• [Journal Article] Japanese Mental Retardation Consortium、Concomitant microduplications of MECP2 and ATRX in male patients with severe mental retardation (2012)
  • Author(s): Honda S, Satomura S, Hayashi S, Imoto I, Nakagawa E, Goto Y, Inazawa J
  • Journal Title: J Hum Genet Volume: 57(1) Pages: 73-7
  • DOI: doi:10.1038/jhg.2011.131.
  • Peer Reviewed
• [Journal Article] Novel intragenic duplications and mutations of CASK in patients with mental retardation and microcephaly with pontine and cerebellar hypoplasia(MICPCH) (2012)
  • Author(s): Hayashi S, Okamoto N, Chinen Y, Takanashi J, Makita Y, Hata A, Imoto I, Inazawa J
  • Journal Title: Hum Genet Volume: 131(1) Pages: 99-110
  • URL: http://www.springerlink.com/content/t41q770m17509j15/?MUD=MP
  • Peer Reviewed
• [Journal Article] Stabilization of phenotypic plasticity through mesenchymal-specific DNA hypermethylation in cancer cells (2012)
  • Author(s): Kurasawa Y, Kozaki K, Pimkhaokham A, Muramatsu T, Ono H, Ishihara T, Uzawa N, Imoto I, Amagasa T, Inazawa J
  • Journal Title: Oncogene Volume: 31(15) Pages: 1963-74
  • DOI: doi:10.1038/onc.2011.373
  • Peer Reviewed
• [Journal Article] HECT-type ubiquitin ligase ITCH targets lysosomal-associated protein multispanning transmembrane 5(LAPTM5) and prevents LAPTM5-mediated cell death (2011)
  • Author(s): Ishihara T, Inoue J, Kozaki K, Imoto I, Inazawa J
  • Journal Title: J Biol Chem Volume: 286(51) Pages: 44086-94
  • URL: http://www.jbc.org/content/286/51/44086.long
  • Peer Reviewed
• [Journal Article] miR-152 is a tumor suppressor microRNA that is silenced by DNA hypermethylation in endometrial cancer (2011)
  • Author(s): Tsuruta T, Kozaki K, Uesugi A, Furuta M, Hirasawa A, Imoto I, Susumu N, Aoki D, Inazawa J
  • Journal Title: Cancer Res Volume: 71(20) Pages: 6450-62
  • URL: http://cancerres.aacrjournals.org/content/71/20/6450.long
  • Peer Reviewed
• [Journal Article] The tumor suppressive microRNA miR-218 targets the mTOR component Rictor and inhibits AKT phosphorylation in oral cancer (2011)
  • Author(s): Uesugi A, Kozaki K, Tsuruta T, Furuta M, Morita K, Imoto I, Omura K, Inazawa J
  • Journal Title: Cancer Res Volume: 71(17) Pages: 5765-78
  • URL: http://cancerres.aacrjournals.org/content/71/17/5765.long
  • Peer Reviewed
• [Book] メディカルサイエンス遺伝子検査学(IV染色体検査法-1染色体分染法-2染色法) (2011)
  • Author(s): 井本逸勢
  • Total Pages: 69-72
  • Publisher: 近代出版
• [Remarks] ホームページ
  • URL: http://plaza.umin.ac.jp/awahg/
• [Remarks] 徳島大学・大学院ヘルスバイオサイエンス研究部・人類遺伝学
  • URL: http://www.tokushima-u.ac.jp/med/culture/jinruiiden/