2012 Fiscal Year Final Research Report
Development of advanced in vivo EMT imaging system and its application for the study of cancer metastasis
| Project/Area Number |
23650600
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Ehime University |
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Principal Investigator |
IMAMURA Takeshi 愛媛大学, 大学院・医学系研究科, 教授 (70264421)
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| Project Period (FY) |
2011 – 2012
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| Keywords | バイオテクノロジー / シグナル伝達 / 遺伝子 / 生体分子 / 細胞・組織 |
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| Research Abstract |
In order to visualize Epithelial to Mesenchymal Transition (EMT) in vivo, I tried to develop a fluorescent probe for EMT by combination ofEMT-specific promoters and a Bimolecular Fluorescence Complementation (BiFC) method. I showed that a BiFC method worked in vivo using LZA and LZB in cells. Moreover, I established an in vivo imaging system for mKO, mCherry, CFP and Venus in living mice. Finally, I screened EMT related promoters and showed that promoters for twist and snail are good candidate for the system.
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