Establishment of protocol for building up monoclonal antibodies targeting cancer stem cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650608
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor immunology
• Research Institution: Okayama University
• Principal Investigator: SAKAGUCHI Masakiyo 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (70379840)
• Co-Investigator(Kenkyū-buntansha): KATAOAKA Ken 岡山理科大学, 理学部, 准教授 (10293317) ; MURATA Hitoshi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90579096) ; HUH Namho 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70142023)
• Project Period (FY): 2011 – 2012
• Keywords: がん幹細胞 / 抗体 / 膜タンパク質 / 腫瘍免疫

Research Abstract

In this study, we succeeded to establish a cancer stem cell (CSC) clone by the innovative plasmid construct. Now, we are in the process of immunization of functional membrane proteins derived from the established CSC clone to generate a panel of monoclonal antibodies against CSCs.

Research Products

• [Journal Article] Coxsackie and adenovirus receptor is a critical regulator for the survival and growth of oral squamous carcinoma cells (2013)
  • Author(s): Saito K, Sakaguchi M, Iioka H, Matsui M, Nakanishi H, Huh NH, Kondo E
  • Journal Title: Oncogene Volume: (in press)
  • DOI: doi:10.1038/onc.2013.66
  • Peer Reviewed
• [Journal Article] DOCK7 is a critical regulator of the RAGE-Cdc42 signaling axis that induces formation of dendritic pseudopodia in human cancer cells (2013)
  • Author(s): Yamamoto K, Murata H, Putranto EW, Kataoka K, Motoyama A, Hibino T, Inoue Y, Sakaguchi M, Huh NH
  • Journal Title: Oncol Rep Volume: 29 Pages: 1073-1079
  • Peer Reviewed
• [Journal Article] S100A9 is a novel ligand of EMMPRIN that promotes melanoma metastasis (2013)
  • Author(s): Hibino T, Sakaguchi M, Miyamoto S, Yamamoto M, Motoyama A, Hosoi J, Shimokata T, Ito T, Tsuboi R, Huh NH
  • Journal Title: Cancer Res Volume: 73 Pages: 172-183
  • Peer Reviewed
• [Journal Article] Preclinical biodistribution and safety study of reduced expression in immortalized cells/Dickkopf-3-encoding adenoviral vector for prostate cancer gene therapy (2012)
  • Author(s): Sugimoto M, Watanabe M, Kaku H, Li SA, Noguchi H, Ueki H, Sakaguchi M, Huh NH, Nasu Y, Kumon H
  • Journal Title: Int J Oncol Volume: 28 Pages: 1645-1652
  • Peer Reviewed
• [Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer (2012)
  • Author(s): Hirata T, Watanabe M, Kaku H, Kobayashi Y, Yamada H, Sakaguchi M, Takei K, Huh NH, Nasu Y, Kumon H
  • Journal Title: Int J Oncol Volume: 41 Pages: 559-564
  • Peer Reviewed
• [Journal Article] S100A7 promotes the migration and invasion of osteosarcoma cells via the receptor for advanced glycation end products (2012)
  • Author(s): Kataoka K, Ono T, Murata H, Morishita M, Yamamoto K, Sakaguchi M, Huh NH
  • Journal Title: Oncol Lett Volume: 3 Pages: 1149-1153
  • Peer Reviewed
• [Journal Article] Preclinical Safety and Efficacy of in Situ REIC/Dkk-3 Gene Therapy for Prostate Cancer (2012)
  • Author(s): Kawauchi K, Watanabe M, Kaku H, Huang P, Sasaki K, Sakaguchi M, Ochiai K, Huh NH, Nasu Y, Kumon H
  • Journal Title: Acta Med Okayama Volume: 66 Pages: 7-16
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in mouse squamous epithelia (2012)
  • Author(s): Kataoka K, Du G, Maehara N, Murata H, Sakaguchi M, Huh N
  • Journal Title: Clin Exp Dermatol Volume: 37 Pages: 428-431
  • Peer Reviewed
• [Journal Article] Partial sensitization of human bladder cancer cells to a gene-therapeutic adenovirus carrying REIC/Dkk-3 by downregulation of BRPK/PINK1 (2012)
  • Author(s): Jin Y, Murata H, Sakaguchi M, Kataoka K, Watanabe M, Nasu Y, Kumon H, Huh NH
  • Journal Title: Oncol Rep Volume: 27 Pages: 695-699
  • Peer Reviewed
• [Journal Article] Tumor suppressor REIC/Dkk-3 interacts with the dynein light chain, Tctex-1 (2011)
  • Author(s): Ochiai K, Watanabe M, Ueki H, Huang P, Fujii Y, Nasu Y, Noguchi H, Hirata T, Sakaguchi M, Huh NH, Kashiwakura Y, Kaku H, Kumon H
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 391-395
  • Peer Reviewed
• [Journal Article] TIRAP, an Adaptor Protein for TLR2/4, Transduces a signal from RAGE Phosphorylated upon Ligand Binding (2011)
  • Author(s): Sakaguchi M, Murata M, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH
  • Journal Title: PLoS ONE Volume: 6 Pages: e23132
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of microRNA-34b/c plays an important role in the pathogenesis of malignant pleural mesothelioma (2011)
  • Author(s): Kubo T, Toyooka S, Tsukuda K, Sakaguchi M, Fukazawa T, Soh J, Asano H, Ueno T, Muraoka T, Yamamoto H, Nasu Y, Kishimoto T, Pass HI, Matsui H, Huh NH, Miyoshi S
  • Journal Title: Clin Cancer Res Volume: 17 Pages: 4965-4974
  • Peer Reviewed
• [Journal Article] Markedly elevated serum levels of calcium-binding S100A8/A9 proteins in psoriatic arthritis are due to activated monocytes/macrophages (2011)
  • Author(s): Aochi S, Tsuji K, Sakaguchi M, Huh NH, Tsuda T, Yamanishi K, Komine M, Iwatsuki K
  • Journal Title: J Am Acad Dermatol Volume: 64 Pages: 879-887
  • Peer Reviewed
• [Journal Article] Intraperitoneal administration of an adenovirus vector carrying REIC/Dkk-3 suppresses peritoneal dissemination of scirrhous gastric carcinoma (2011)
  • Author(s): Than SS, Kataoka K, Sakaguchi M, Murata H, Abarzua F, Taketa C, Du G, Yashiro M, Yanagihara K, Nasu Y, Kumon H, Huh NH
  • Journal Title: Oncol Rep Volume: 25 Pages: 989-995
  • Peer Reviewed
• [Journal Article] A new cytosolic pathway from a Parkinson's disease-associated kinase, BRPK/PINK1: activation of AKT via MTORC2 (2011)
  • Author(s): Murata H, Sakaguchi M, Jin Y, Sakaguchi Y, Futami JI, Yamada H, Kataoka K, Huh NH
  • Journal Title: J Biol Chem Volume: 286 Pages: 7182-7189
  • Peer Reviewed
• [Journal Article] Expression of REIC/Dkk-3 in normal and hyperproliferative epidermis (2011)
  • Author(s): Du G, Kataoka K, Sakaguchi M, Abarzua F, Than SS, Sonegawa H, Makino T, Shimizu T, Huh NH
  • Journal Title: Exp Dermatol Volume: 20 Pages: 273-277
  • Peer Reviewed
• [Presentation] Identification of novel receptors for pro-inflammatory S100A8/A9 proteins and their roles inmelanoma metastasis (2012)
  • Author(s): 阪口政清
  • Organizer: IEIIS2012. Homeostatic Inflammation Symposium
  • Place of Presentation: 東京
  • Year and Date: 20121023-26
• [Presentation] S100A8 および S100A9 タンパク質の新規受容体の探索とそのがん進展における役割 (2012)
  • Author(s): 阪口政清
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] A Novel Tumor Suppressor, REIC/Dkk-3 Gene Identified by our in Vitro Transformation Model of Normal Human Fibroblasts Works as a Potent Therapeutic Anti-tumor Agent (2012)
  • Author(s): 阪口政清
  • Organizer: 2012 World Congress on In Vitro Biology
  • Place of Presentation: アメリカ、Bellevue(招待講演)
  • Year and Date: 20120603-07
• [Presentation] 多機能受容体 RAGE の下流信号伝達機構の解明 (2011)
  • Author(s): 阪口政清
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Patent(Industrial Property Rights)] NPTNβとS100A8の結合の阻害を指標とする細胞増殖抑制剤のスクリーニング方法 (2012)
  • Inventor(s): 阪口政清、日比野利彦、山本真実、許南浩
  • Industrial Property Rights Holder: 阪口政清、日比野利彦、山本真実、許南浩
  • Industrial Property Number: 特願2012-204279
  • Filing Date: 2012-09-18
• [Patent(Industrial Property Rights)] PINK1のユビキチンアッセイ及びスクリーニングへの利用 (2012)
  • Inventor(s): 村田 等、阪口政清、許南浩
  • Industrial Property Rights Holder: 村田 等、阪口政清、許南浩
  • Industrial Property Number: 特許、特願 2012-165160
  • Filing Date: 2012-07-25
• [Patent(Industrial Property Rights)] アデノウイルスベクターをがん細胞に対して選択的に導入可能なポリペプチドおよび当該ポリペプチドを備えるアデノウイルスベクター (2012)
  • Inventor(s): 阪口政清、近藤英作、許南浩、手塚克成
  • Industrial Property Rights Holder: 阪口政清、近藤英作、許南浩、手塚克成
  • Industrial Property Number: 特願2012-085969
  • Filing Date: 2012-04-04