Non-linear DNA repair responses caused by interference between competitive repair pathways

2013 Fiscal Year Final Research Report

• Project/Area Number: 23651049
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: YOKOYA Akinari 独立行政法人日本原子力研究開発機構, 原子力科学研究部門・先端基礎研究センター, 研究主席 (10354987)
• Project Period (FY): 2011 – 2012
• Keywords: クラスターDNA損傷 / DNA修復応答 / 酵素反応干渉 / 非線形性

Research Abstract

Ionizing radiation induce localized DNA damage composed of various base lesions (clustered damage). It is expected that damage repair enzymes would be interfered at the localized area on DNA. In this study we aimed to clarify whether the variation of enzymatic processes modify each enzymatic activities at clustered damage site. DNA samples exposed to X-rays were treated with two base excision repair enzymes, namely Nth and Fpg. The enzymes were added sequentially or simultaneously. Contrast to our initial expectation, change of the order of the enzymatic treatments did not affect the activities of the enzymes. This result suggests that, even in a cluster damage site, each base lesions substantially separated not to induce interference between each enzymatic pathways.

Research Products

• [Journal Article] Order effect of repair processes to clustered DNA damage (2014)
  • Author(s): Shiraishi, I., Suzuki, M., Shikazono, N., Fujii, K. Yokoya, A
  • Journal Title: J. Radiat. Res Volume: 55, Suppl1 Pages: i92-i93
  • DOI: 10.1093/jrr/rrt152
• [Journal Article] Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation (2013)
  • Author(s): Shiina, T., Watanabe, R., Shiraishi, I., Suzuki, M., Sugaya, Y., Fujii, K. and Yokoya, A
  • Journal Title: Radiat. Environ. Biophys Volume: 52 Pages: 99-112
  • Peer Reviewed
• [Journal Article] Yield of single-, double-strand breaks and nucleobase lesions in fully hydrated plasmid DNA films irradiated with high-LET charged particles (2012)
  • Author(s): Ushigome, T., Shikazono, N., Fujii, K., Watanabe, R., Suzuki, M., Tsuruoka, C., Tauchi, H. and Yokoya, A
  • Journal Title: Radiat. Res Volume: 177 Pages: 614-627
  • Peer Reviewed
• [Journal Article] A novel technique using DNA denaturation to detect multiply induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and 4He2+ ion irradiation (2011)
  • Author(s): Yokoya, A., Shikazono, N., Fujii, K., Noguchi, M. and Urushibara, A
  • Journal Title: Radiat. Protect. Dosim Volume: 143 Pages: 219-225
  • Peer Reviewed
• [Presentation] Molecular and Cellular Effect of Ionizing Radiation (2013)
  • Author(s): Yokoya, A., Oka, T., Fujii, K., Shiina, T., Sugaya, Y., Shiraishi, I., Urushibara, A., Narita1, A., Noguchi, M. and Watanabe, R
  • Organizer: The 16th SANKEN International The 11th SANKEN Nanotechnology Symposium
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20130122-23
  • Invited
• [Presentation] DNA 分子上の放射線損傷分布とDNA 損傷修復経路干渉の相関 (2012)
  • Author(s): 白石伊世, 椎名卓也, 菅谷雄基, 鹿園直哉, 横谷明徳
  • Organizer: 日本放射線影響学会第55回大会
  • Place of Presentation: 仙台市
  • Year and Date: 20120906-08
• [Presentation] Order effect of base excision processes to repair clustered DNA damage (2012)
  • Author(s): Shiraishi, I., Shikazono, N. and Yokoya, A
  • Organizer: The 12th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 20120602-06
• [Presentation] 放射線によるゲノムDNA 損傷の初期過程と生体修復 (2012)
  • Author(s): 横谷明徳
  • Organizer: 日本物理学会第67回年次大会
  • Place of Presentation: 西宮市
  • Year and Date: 20120324-27
  • Invited
• [Book] Chapter 20. Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair. In : Charged particle and photon interactions with matter-recent advances, applications and interfaces, Eds., Y. Hatano, Y. Katsumura, and A. Mozumder (2011)
  • Author(s): Yokoya, A., Fujii, K., Shikazono, N. and Ukai, M
  • Total Pages: 543-574
  • Publisher: CRC/Taylor & Francis Group, USA
• [Remarks]
  • URL: http://asrc.jaea.go.jp/soshiki/gr/yokoya-gr/