2013 Fiscal Year Final Research Report
A novel study to identify DNA determinants that generate the meiotic recombination hotspots
| Project/Area Number |
23651185
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
OHYAMA Takashi 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 減数分裂期組換え / 高頻度組換え部位 / DSBホットスポット / 相同組換え / 出芽酵母ゲノム / 分裂酵母ゲノム / マウスゲノム / 二本鎖DNA崩壊エネルギー |
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| Research Abstract |
We explored characteristics of the physical properties and conformations of DSB (double-strand break) hotspots on the genomes of yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and mouse (Mus musculus). When averaged, DSB hotspots of each organism clearly showed a distinctive mechanical property compared to the surrounding regions. However, very interestingly, the properties themselves were opposite between the yeasts and mouse: i.e., DSB hotspots are very rigid in the former while very flexible in the latter. Another physical property to be noted was that DSB hotspots of all the three organisms show distinctively high duplex disruption energy compared to the surrounding regions.
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Research Products
(11 results)
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[Journal Article] Regions with unusually high flexibility occur frequently in human genomic DNA2013
Author(s)
Kimura, H., Kageyama, D., Furuya, M., Sugiyama, S., Murata, N. and Ohyama, T
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Journal Title
Biosci. Biotechnol. Biochem
Volume: 77
Pages: 612-617
DOI
Peer Reviewed
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[Journal Article] The genome folding mechanism in yeast2013
Author(s)
Kimura, H., Shimooka, Y., Nishikawa, J., Miura, O., Sugiyama, S., Yamada, S. and Ohyama, T
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Journal Title
J. Biochemistry
Volume: 154
Pages: 137-147
DOI
Peer Reviewed
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