2011 Fiscal Year Final Research Report
Development of the new detection method for 5-hydroxymethylcytidine in genomic DNA
| Project/Area Number |
23651188
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HANAMI Takeshi 独立行政法人理化学研究所, LSA要素技術開発ユニット, 研究員 (60392073)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Masayoshi 独立行政法人理化学研究所, オミックス制御研究ユニット, ユニットリーダー (90344027)
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| Project Period (FY) |
2011
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| Keywords | 5-ヒドロキシメチルシチジン / 化学変換 / 配列決定 |
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| Research Abstract |
DNA oligomer containing 5-hmdC is react with protecting reagent with solvation to the aprotic solvent in the presence of long chain alkyl ammonium salt. The result showed that 5-hmdC is protected selectively by bridging 4'-amine and 5'-hydroxymethyl group. This protection group is stable under the condition of deamination reaction.
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