2012 Fiscal Year Final Research Report
Elucidation of signaling pathway with visualization of redox stateduring nitrogen starvation and diseases accompanying intra-cellular reduction
| Project/Area Number |
23651235
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Kyoto University |
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Principal Investigator |
SAKAI Yasuyoshi 京都大学, 大学院・農学研究科, 教授 (60202082)
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| Project Period (FY) |
2011 – 2012
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| Keywords | レドックス / 還元化 / ペルオキシソーム / FRET / チオレドキシン |
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| Research Abstract |
In this project we aimed to elucidate the mechanism of intra-cellular reduction in model cell lines of a hereditary disease or in yeast cells exposed to nutrientstarvation (including nitrogen starvation), by utilizing a sensor protein of intra-cellular redox state termed Redoxfluor which we had developed. As a result we found that intra-cellular reduction observed in CHO cell lines of peroxisomal disease had a correlation with the level of very-long chain fatty acid. Furthermore, we discovered that thioredoxin proteins are important for the maintenance of cellular redox in yeast cells exposed to both heat stress and nutrient-deprivation.
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