2012 Fiscal Year Final Research Report
DEVELOPMENT OF ON/OFF FLUORESCENT PROBES FOR PROTEINS BASED ON RESTRICTION OF INTRAMOLECULAR ROTATION
| Project/Area Number |
23651242
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 蛍光 / 蛍光センシング / BODIPY / 炭酸脱水酵素 / カナマイシン / カナマイシン修飾酵素 / カナマイシン耐性菌 / ^19F-NMR |
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| Research Abstract |
Phenyl-BODIPY probes having an enzyme inhibitor or enzyme substrate moiety as a substituent binds to the corresponding enzyme, thereby undergoing restriction of internal rotation of the probe to result in enhanced fluorescence. 19F-NMR indicates that internal rotation in concern is indeed inhibited in the probes subject to complexation-induced fluorescence enhancement. The present tool is applicable to a wide range of proteins (enzymes and receptors) and that bind to a small molecules and is characteristically versatile in that respect.
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