2013 Fiscal Year Final Research Report
Constructing cDNA for hypothesis verification of crystal melting of Ca2+driven contractile protein
| Project/Area Number |
23654153
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics/Chemical physics
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| Research Institution | Waseda University |
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Principal Investigator |
ASAI Hiroshi 早稲田大学, 理工学術院, 名誉教授 (70063584)
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| Co-Investigator(Renkei-kenkyūsha) |
ASAHI Toru (80222595)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 生物物理 |
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| Research Abstract |
1)In order to analyze peptide sequences of a putative protein of spaconnectin which considered to have a co-operative contractile role with the another protein (spasmin), Thus, we intended to collect the peritrich ciliate (Zoothamnium arbuscula strain Lake Biwa) at the shallow water around the Karasuma Peninsula at Lake Biwa. The physiological role of this spaconnectin is supposed to be entropic contraction of spasmoneme.2) In this year, we could get abundant amounts of the organisms. And We have also collected and incubated some amounts of green vorticella and other species for determining small subunit RNA genes in pure ethanol. 3)We intended to investigate if animal erythrocyte have properties of Ca2-stimulative contraction similarly as vorticellae stalk. Cow erythrocyte was tested. Result of research was obscure so the the Jenopus erythrocyte was investigated. The result was successful. It means that the erythrocyte contracted at about 10µ amounts of Ca2+.
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