2013 Fiscal Year Final Research Report
Nanoscale imaging of biocatalytic activity with redox-responsive fluorescent probes
| Project/Area Number |
23655009
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 蛍光イメージング / 単一分子 / 触媒 |
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| Research Abstract |
Nano-sized catalysts such as enzymes and inorganic nanoparticles have both potential and demonstrated applications for use in (bio)chemical synthesis and energy production. In this study, we developed new fluorescence probes for molecular adsorption and interfacial electron transfer reactions on individual nanocatalysts. The synthesized BODIPY-based fluorescence probes, which have one or two sulfonate groups and a dinitrophenyl group as a redox reaction site, were used to investigate the photoinduced electron transfer reactions over catalysts at the single-particle, single-molecule levels. We also applied single-molecule-based super-resolution fluorescence microscopy with catechol-modified BODIPY to identify the locations of effective adsorption sites on metal oxide surfaces.
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