2013 Fiscal Year Final Research Report
Development of a long-term culture system for functional Sertoli cells.
| Project/Area Number |
23659095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto University |
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Principal Investigator |
SHINOHARA Takashi 京都大学, 医学(系)研究科(研究院), 教授 (30322770)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 精子形成 |
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| Research Abstract |
It has not been possible to culture functional Sertoli cells for long-term. We have developed a new system for culturing mouse Sertoli cells that provide microenvironment for spermatogonial stem cells. In this culture system, Sertoli cells could maintain spermatogonial stem cell activity for more than five months when they were supplemented with epidermal growth factor and fibroblast growth factor 2. Spermatogonial stem cells migrated under the Sertoli cell feeder layer and formed cobblestone colonies. Cultured spermatogonial stem cells could produce functional sperm upon transplantation into the seminiferous tubules of infertile mouse testes. Because cobblestone colony formation was suppressed by inhibiting CXCR4 or RET, these molecules may be involved in migration of spermatogonial stem cells to germline niche. Taken together, our novel culture system will provide a new system for functional assay of spermatogonial stem cells.
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[Remarks] 新聞での報道 2011年11月5日 京都新聞朝刊 27面京大グループ 精子幹細胞の増殖に働くタンパク質特定 移植治療への応用に期待
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[Remarks] 新聞での報道 2012年10月5日 京都新聞朝刊 24面精子幹細胞 増殖環境を再現-京大など, 試験管内に 移植治療に応用期待
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[Remarks] 新聞での報道 2012年10月5日 産經新聞朝刊 23面精巣内定着率向上 タンパク質特定 京大グループ
