2013 Fiscal Year Final Research Report

Analyses on possible roles that nutrient transporters can play in the postnatal development of murine cerebellar cortex.

Research Project

Project/Area Number	23659103
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	General anatomy (including Histology/Embryology)
Research Institution	Teikyo University
Principal Investigator	ABE Hiroyuki 帝京大学, 医療技術学部, 講師 (80309335)
Project Period (FY)	2011 – 2013
Keywords	トランスポーター / GLUT1 / PiT1 / PiT2 / 小脳 / 発達
Research Abstract	Present research focuses on studying possible roles that nutrient transporters can play in the postnatal development of murine cerebellar cortex. First, we established a simple method to inject reagents into the cerebellum of newborn mouse in order to suppress the expression of nutrient transporters based on the activity of the injected reagent, and presented the achievements in the 61st. Annual Meeting of Japanese Association for Laboratory Animal Science (Sapporo, JAPAN) (manuscript in preparation). Next, we designed a reagent that specifically suppresses the expression of a nutrient transporter based on the nucleotide sequence of its gene, and confirmed its effects on a murine cultured cell line. This reagent will be injected in the cerebellum of newborn mouse, and its possible effects on the postnatal development of cerebellar cortex will be studied.