The genetic immunotherapy by novel nasal spray vaccine for melanomausing the next generation type virus vector

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659549
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Mie University
• Principal Investigator: KAWANO Mitsuo 三重大学, 大学院・医学系研究科, 講師 (00234097)
• Co-Investigator(Kenkyū-buntansha): YAMANAKA Keiichi 三重大学, 医学系研究科, 准教授 (70314135)
• Project Period (FY): 2011 – 2012
• Keywords: 皮膚腫瘍学

Research Abstract

To establish a novel genetic immunotherapy instead of conventional chemotherapy and radiotherapy against refractory malignant melanoma, we have developed the parainfluenza type 2 virus vector introduced TRP-2 gene known as melanoma differentiation antigen or Ag85B gene inducing strong Th1 adjuvant activity. We analyzed the antitumor effect and the immune mechanisms by intranasal administrationto melanoma like model mice using the next generation spray vaccine for melanoma.

Research Products

• [Journal Article] Single systemic administration of Ag85B of mycobacteria DNA inhibits allergic airway inflammation in a mouse model of asthma (2012)
  • Author(s): Karamatsu K, Matsuo K, Inada H, Tsujimura Y, Shiogama Y, Matsubara A, Kawano M, Yasutomi Y
  • Journal Title: J Asthma Allergy Volume: 5 Pages: 71-79
  • DOI: DOI:10.2147/JAA.S37667
  • Peer Reviewed
• [Journal Article] Ustekinumab improves psoriasis without altering T cell cytokine production, differentiation, and T cell receptor repertoire diversity (2012)
  • Author(s): Tsuda K, Yamanaka K, Kondo M, Matsubara K, Sasaki R, Tomimoto H, Gabazza EC, Mizutani H
  • Journal Title: PLoS One Volume: 7(12) Pages: e51819
  • DOI: DOI:10.1371/journal.pone. 0051819
  • Peer Reviewed
• [Journal Article] Legume lectins inhibit human parainfluenza virus type 2 infection by interfering with the entry (2012)
  • Author(s): Uematsu J, Koyama A, Takano S, Ura Y, Tanemura M, Kihira S, Yamamoto H, Kawano M, Tsurudome M, O'Brien M, Komada H
  • Journal Title: Viruses Volume: 4(7) Pages: 1104-15
  • DOI: DOI:10.3390/v4071104
  • Peer Reviewed
• [Journal Article] Calcineurin inhibitors suppress cytokine production from memory T cells and differentiation of naive T cells into cytokine-producing mature T cells (2012)
  • Author(s): Tsuda K, Yamanaka K, Kitagawa H, Akeda T, Naka M, Niwa K, Nakanishi T, Kakeda M, Gabazza EC, Mizutani H
  • Journal Title: PLoS One Volume: 7(2) Pages: e31465
  • DOI: DOI:10.1371/journal.pone.0031465
  • Peer Reviewed
• [Journal Article] Heat-killed bacillus Calmette-Guerin and Mycobacterium kansasii antigen 85B combined vaccination ameliorates dermatitis in a mouse model of atopic dermatitis by inducing regulatory T cells (2012)
  • Author(s): Kakeda M, Yamanaka K, Kitagawa H, Tsuda K, Akeda T, Kurokawa I, Gabazza EC, Mizutani H
  • Journal Title: Br J Dermatol Volume: 166(5) Pages: 953-63
  • DOI: DOI:10.1111/j.1365-2133.2011.10763.x
  • Peer Reviewed
• [Journal Article] Identification of domains on the fusion (F) protein in trimer that influence the hemagglutinin-neuraminidase specificity of the f protein in mediating cell-cell fusion (2011)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y
  • Journal Title: J Virol Volume: 85(7) Pages: 3153-3161
  • DOI: DOI:10.1128/JVI.01666-10
  • Peer Reviewed
• [Presentation] リバビリンによるヒトパラインフルエンザウイルス2型増殖阻害に対するニトロベンジルチオイノシン、アクチノマイシンD及びグアノシンの中和作用 (2013)
  • Author(s): 紀平佐保子、植松淳、山本秀孝、河野光雄、鶴留雅人、駒田洋
  • Organizer: 日本薬学会133年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2013-03-30
• [Presentation] カテキン混合物及びタンニン酸によるパラインフルエンザウイルス2型増殖阻害 (2013)
  • Author(s): 山本秀孝、内田美香、坂倉百合香、出口小百合、紀平佐保子、植松淳、河野光雄、鶴留雅人、駒田洋
  • Organizer: カテキン混合物及びタンニン酸によるパラインフルエンザウイルス2型増殖阻害
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2013-03-30
• [Presentation] パラミクソウイルスの膜融合誘導機構:二分子蛍光相補性(BiFC)法を用いた受容体結合蛋白と膜融合蛋白の相互作用の解析 (2012)
  • Author(s): 鶴留雅人、大塚順平、駒田洋、河野光雄、伊藤守弘、野阪哲哉
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2012-11-15
• [Presentation] 漢方薬によるパラインフルエンザウイルス2型増殖阻害 (2012)
  • Author(s): 山本秀孝、伊藤愛、前田友里恵、大河内愛弓、佐藤冴美、紀平佐保子、植松淳、河野光雄、鶴留雅人、駒田洋
  • Organizer: 日本薬学会 第132年会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-30
• [Presentation] リバビリンによるパラインフルエンザ2型増殖阻害 (2012)
  • Author(s): 紀平佐保子、大河内愛弓、佐藤冴美、伊藤愛、前田友里恵、河野光雄、鶴留雅人、植松淳、山本秀孝、駒田洋
  • Organizer: 日本薬学会 第132会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-29
• [Presentation] Identification of amino acids of simian virus 41 (SV41) fusion protein that convert parainfluenza virus 5 fusion protein to a protein which specifically interacts with SV41 hemagglutininneuraminidase by substitution. (2011)
  • Author(s): Masato Tsurudome, Mito Nakahashi, Yoshiaki Matsushima, Machiko Nishio, Mitsuo Kawano, Hiroshi Komada, Tetsuya Nosaka
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center (Sapporo, Hokkaido)
  • Year and Date: 2011-09-15