Proteomic analysis of extracellular matrix proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 23659727
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokushima
• Principal Investigator: SATO Nori 徳島大学, 大学病院, 医員 (00448333)
• Co-Investigator(Kenkyū-buntansha): YASUI Natsuo 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (00157984) ; HIGASHINO Kosaku 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (80380129) ; GODA Yuichiro 徳島大学, 病院, 助教 (70581148)
• Co-Investigator(Renkei-kenkyūsha): TANIGUCHI Hisaaki 徳島大学, 疾患酵素学研究センター, 教授 (10257636)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 細胞外マトリックス / コラーゲン / エラスチン / 質量解析 / 不溶性 / 電子顕微鏡 / 腰部脊柱管狭窄症

Outline of Final Research Achievements

Tendons and ligaments are mostly composed of extracellular matrix. These tissues are insoluble, and any study in whole proteomic analysis of them has not yet been reported. We succeeded in complete solubilization of human Achilles tendon and yellow ligament for HPLC-ESI-MS/MS. Our method will be applicable to investigation on protein composition of other extracellular matrix such as bone, cartilage, vessel, skin and so on, and it will be helpful to reveal the pathophysiology of pathological them.

Free Research Field

整形外科学