The molecular analysis of placental abruption using in vitro placental model

2013 Fiscal Year Final Research Report

• Project/Area Number: 23659779
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: KIMURA Tadashi 大阪大学, 医学(系)研究科(研究院), 教授 (90240845)
• Co-Investigator(Kenkyū-buntansha): SAWADA Kenjiro 大阪大学, 医学系研究科, 講師 (00452392) ; MABUCHI Seiji 大阪大学, 医学系研究科, 助教 (00452441) ; ISOBE Aki 大阪大学, 医学系研究科, 助教 (60397619) ; HASHIMOTO Kae 大阪大学, 医学系研究科, 助教 (90612078)
• Project Period (FY): 2011 – 2013
• Keywords: 常位胎盤早期剥離 / 前期破水 / インターロイキン / プロスタグランジン / 絨毛羊膜炎

Research Abstract

Intrauterine infection is a key trigger of placental abruption. In order to analyze the potential role of inflammatory cytokines in this pathogenesis, we examined the expressional pattern of various cytokines in placentae complicated with severe chorioamnionitis (CAM) and found that IL-6 is mainly expressed in macrophages in villous mesenchyme. Using an experimental lipopolysaccharide (LPS)-induced PTD model, the therapeutic potential of targeting this cytokine was investigated. Anti-IL-6 receptor antibody (MR16-1) was delivered before LPS treatment. Mice in the MR16-1 group had a significantly lower rate of PTD (17%) than 53% in the controls. As a result, MR16-1 treatment significantly prolonged the gestational period without any apparent adverse events on the mice and their pups. Thus, targeting IL-6 signaling can be a promising option for the prevention of placental abruption and needs to be further explored for future clinical application.

Research Products

• [Journal Article] Targeting interleukin-6 receptor inhibits preterm delivery induced by inflammation (2013)
  • Author(s): Wakabayashi, A. Sawada, K. Nakayama, M. Toda, A. Kimoto, A. Mabuchi, S. Kinose, Y. Nakamura, K. Takahashi, K. Kurachi, H. Kimura, T
  • Journal Title: Mol Hum Reprod Volume: 19 Pages: 718-726
• [Presentation] 感染性早産に対するIL‐6 に焦点を当てた分子標的治療の可能性の検討 (2013)
  • Author(s): 澤田健二郎
  • Organizer: 第21回日本胎盤学会
  • Place of Presentation: 名古屋
  • Year and Date: 20131025-26
• [Presentation] 卵巣癌組織におけるインターロイキン6(IL-6)とその受容体の発現が予後に与える影響の解析 (2013)
  • Author(s): 磯部 晶. 澤田健二郎. 小倉寛則. 木瀬康人. 川島英理子. 牧野 弘. 水野智子.木村 正. 森重健一郎
  • Organizer: 第65回日本産科婦人科学会
  • Place of Presentation: 札幌
  • Year and Date: 20130510-12