Development of a novel quality assurance system for bone marrow stromal cells using morphology-based prediction.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659912
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: KAGAMI Hideaki 松本歯科大学, 歯学部, 教授 (80242866)
• Co-Investigator(Kenkyū-buntansha): KATO Ryuji 名古屋大学, 創薬科学研究科, 准教授 (50377884)
• Project Period (FY): 2011 – 2012
• Keywords: 移植・再生医療 / 細胞・組織 / トランスレーショナルリサーチ / 再生医学 / 情報工学

Research Abstract

The purpose of this study was to test the feasibility of morphology-based prediction system of osteogenic differentiation. First, cell morphology was continuously recorded and analyzed with a latest computed system. These data were compared with the results from biochemical assays and potential indices to predict cell differentiation status and functional abnormality were selected. A software was developed using those indices.

Research Products

• [Journal Article] Characteristic differences among osteogenic cell populations of rat bonemarrow stromal cells isolated from untreated, hemolyzed or Ficoll-treated marrow. (2012)
  • Author(s): Agata H, Yamazaki M, Uehara M, Hori A, Sumita Y, Tojo A, Kagami H.
  • Journal Title: Cytotherapy Pages: 791-801
  • DOI: doi:10.3109/14653249.2012.674639
  • Peer Reviewed
• [Journal Article] Effect of GDF-5 and BMP-2 on the expression of tendo/ligamentogenesis-related markers in human PDL-derived cells. (2012)
  • Author(s): Inoue M, Ebisawa K, Itaya T, Sugito T, Yamawaki-Ogata A, Sumita Y, Wadagaki R, Narita Y, Agata H, Kagami H, Ueda M.
  • Journal Title: Oral Dis Volume: 18 Pages: 206-212
  • Peer Reviewed
• [Journal Article] their similarities and differences revealed from gene expression analyses. (2011)
  • Author(s): Ebisawa K, Kato R, Sugimura T, LatifMA, Hori Y, Narita Y, Ueda M, Honda H, Kagami H. Gingival and dermal fibroblasts
  • Journal Title: J Bioscien Bioeng Volume: 111 Pages: 255-258
  • DOI: doi:10.1016/j.jbiosc.2010.11.014..
  • Peer Reviewed
• [Journal Article] Bone marrow stromal cells (bonemarrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation. (2011)
  • Author(s): Kagami H., Agata H, Tojo A.
  • Journal Title: Int J. Biochem. Cell Biol. Volume: 43 Pages: 286-289
  • DOI: doi:10.1016/j.biocel.2010.12.006..
  • Peer Reviewed
• [Journal Article] A compact, automated cell culture system for clinical scale cell expansion from primary tissues. (2010)
  • Author(s): Kato R, Iejima D, Agata H, Asahina I, Okada K, Ueda M, Honda H, Kagami H.
  • Journal Title: Tissue Eng Part C Methods Volume: 16 Pages: 947-956
  • DOI: doi:10.1089/ten.TEC.2009.0305
  • Peer Reviewed
• [Journal Article] Differential effect of scaffold shape on dentin regeneration. (2010)
  • Author(s): Tonomura A, Mizuno D, Hisada A, Kuno N, Ando Y, Sumita Y, Honda MJ, Satomura K, Sakurai H, Ueda M, Kagami H.
  • Journal Title: Annals Biomed Eng Volume: 38 Pages: 1664-1671
  • DOI: doi:10.1007/s10439-010-9910-z.
  • Peer Reviewed
• [Journal Article] Efficacy of membranous cultured periosteum for the treatment of patients with severe periodontitis : A proof-of-concept study. (2010)
  • Author(s): Mizuno H, Kagami H, Mase J, Mizuno D, Ueda M.
  • Journal Title: Nagoya J Med Sci Volume: 72 Pages: 59-70
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20229704.
  • Peer Reviewed
• [Journal Article] Effects of extracellular matrix on differentiation of human bone marrow-derived mesenchymal stem cells into smooth muscle cell lineageUtility for cardiovascular tissue engineering. (2010)
  • Author(s): Suzuki S, Narita Y, Yamawaki A, Murase Y, Satake M, Mutsuga M, Okamoto H, Kagami H, Ueda M, Ueda Y.
  • Journal Title: Cells Tissues Organs Volume: 191 Pages: 269-280
  • DOI: doi:10.1159/000260061
  • Peer Reviewed
• [Journal Article] Evaluation of scaffold materials fortooth tissue engineering. (2010)
  • Author(s): Ohara T, Itaya T, Usami K, Ando Y, Sakurai H, Honda MJ, Ueda M, Kagami H.
  • Journal Title: J Biomed MaterRes A Volume: 94 Pages: 800-805
  • DOI: doi:10.1002/jbm.a.32749..
  • Peer Reviewed
• [Journal Article] Differential effects of growth differentiation factor-5 on porcine dental papilla- and follicle-derived cells. (2010)
  • Author(s): Sumita Y, Honda MJ, Ueda M, Asahina I, Kagami H.
  • Journal Title: Growth Factors Volume: 28 Pages: 56-65
  • DOI: doi:10.3109/08977190903373380..
  • Peer Reviewed
• [Journal Article] Characteristic change and loss of in vivo osteogenic abilities of human bone marrow stromal cells during passage. Tissue (2010)
  • Author(s): Agata H, Asahina I, Watanabe N, Ishii Y, Kubo N, Ohshima S, Yamazaki M, Tojo A, Kagami H
  • Journal Title: Engineering Part A Volume: 16 Pages: 663-673
  • DOI: doi:10.1089/ten.TEA.2009.0500
  • Peer Reviewed
• [Journal Article] Limited but heterogeneous osteogenic response of human bone marrow mesenchymal stem cells to bone morphogenetic protein-2 and serum. (2010)
  • Author(s): Mizuno M, Agata H, Furue H, Kimura A, Narita Y, Watanabe N, Ishii Y, Ueda M, Tojo A, Kagami H.
  • Journal Title: Growth Factors Volume: 28 Pages: 34-43
  • DOI: doi:10.3109/08977190903326362..
  • Peer Reviewed
• [Journal Article] Recovery process of sciatic nerve defect with novel bioabsorbable collagen tubes packed with collagen filaments in dogs. (2010)
  • Author(s): Okamoto H, Hata K-I, Kagami H, Okada K, Ito Y, Narita Y, Hirata H, Sekiya I, Otsuka T, Ueda M.
  • Journal Title: J Biomed MaterRes A Volume: 92 Pages: 859-868
  • DOI: doi:10.1002/jbm.a.32421..
  • Peer Reviewed
• [Journal Article] The potential of somatic stem cells for alveolar bone tissue engineering. (2010)
  • Author(s): Kagami H, Agata H.
  • Journal Title: Int. J. Oral-Medical Sci. Volume: 9 Pages: 1-10
  • URL: https://mol.medicalonline.jp/archive/search?jo=de1ijoms&vo=9&nu=1.
  • Peer Reviewed
• [Journal Article] Morphology-based prediction of osteogenic differentiation potential of human mesenchymal stem cells.
  • Author(s): Matsuoka F, Takeuchi I, Agata H, Kagami H, Shiono H, Kiyota Y, Honda H, Kato R.
  • Journal Title: Plos One
  • Peer Reviewed
• [Journal Article] Ischemic culture of dental pulp-derived cells is a useful model in which to investigate mechanisms of post-ischemic tissue recovery.
  • Author(s): Agata A, Sumita Y, Asahina I, Tojo A, Kagami H
  • Journal Title: Histol Histopathol
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23629696.
  • Peer Reviewed
• [Presentation] 歯槽骨・歯科における再生医療の将来 (2013)
  • Author(s): 各務秀明、縣秀樹、住田吉慶、朝比奈泉
  • Organizer: 第12回日本再生医療学会総会シンポジウム 5
  • Place of Presentation: 横浜
  • Year and Date: 20130319-0321
• [Presentation] Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation (2013)
  • Author(s): Kagami H.
  • Organizer: 2013 Potential Applications of Mesenchymal Multipotent Stromal Cells. The 16th US-Japan Cellular and Gene Therapy Conference, NIDCR, NIH
  • Year and Date: 2013-02-28
• [Presentation] 骨再生医療の現状と展望について (2012)
  • Author(s): 各務 秀明
  • Organizer: 第57回日本口腔外科学会総会・学術大会シンポジウムI 歯・歯周組織・骨の再生医療
  • Place of Presentation: 横浜
  • Year and Date: 20121019-21
• [Presentation] 自己骨髄由来培養骨芽細胞様細胞による歯槽骨再生スーパー特区:医 工連携による先進医療機器の実用化プロジェクト (2012)
  • Author(s): 各務 秀明
  • Organizer: 第三回シンポジウム、文部科学省橋渡し研究支援推進プログラム:先端医療 の開発支援拠点形成と実践第五回シンポジウム
  • Place of Presentation: 東京大学医学部附属病院
  • Year and Date: 2012-12-28
• [Presentation] 骨髄由来間葉系幹細胞の画像情報解析による骨分化度予測 (2012)
  • Author(s): 加藤竜司
  • Organizer: 第11回 日本再生医療学会総会
  • Year and Date: 2012-06-12
• [Presentation] 再生医療における品質管理の課題:歯槽骨再生治療について 日本臨床 試験研究会 (2012)
  • Author(s): 各務 秀明
  • Organizer: 第3回学術集会総会分科会:再生医療再生医療と規制
  • Place of Presentation: 福岡国際会議場第1会場501室
  • Year and Date: 2012-02-24
• [Presentation] 骨髄細胞を用いた骨再生とインプラント治療 (2012)
  • Author(s): 各務 秀明
  • Organizer: バイオインテグレーション学会第2回学術大会・総会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2012-01-29
• [Presentation] Bone marrow stromal cells (bone marrow-derived multipotent mesenchymal stromal cells) for alveolar bone tissue engineering: basic science to clinical translation (2011)
  • Author(s): Kagami H.
  • Organizer: The Second Chinese National Conference on Oral MaxillofacialDevelopment and Regeneration
  • Place of Presentation: Wuyishan city, Fujian Province, China
  • Year and Date: 20110728-0731
• [Presentation] 歯からはじまるアンチエイジングーインプラントと骨の再生医療ー (2011)
  • Author(s): 各務秀明
  • Organizer: 第12回市民公開医療懇談会
  • Place of Presentation: 東京大学医科学研究所附属病院
  • Year and Date: 2011-07-28
• [Presentation] 実用化を目指した新たな歯槽骨再生臨床研究の開始について (2011)
  • Author(s): 各務 秀明
  • Organizer: 第10回松本ボーンフォーラム
  • Place of Presentation: 松本歯科大学30周年記念棟
  • Year and Date: 2011-05-28
• [Presentation] 自己骨髄由来培養骨芽細胞様細胞による歯槽骨再生 スーパー特区:医 工連携による先進医療機器の実用化プロジェクト (2011)
  • Author(s): 各務 秀明
  • Organizer: 第三回シンポジウム 文部科学省橋渡し研究支援推進プログラム:先端医療 の開発支援拠点形成と実践第五回シンポジウム 2
  • Place of Presentation: 東京大学医学部附属病院
  • Year and Date: 2011-02-28
• [Presentation] ヒト幹細胞指針に基づいた臨床研究とネットワーク構築によるトランスレーショナルリサーチの展開 (2010)
  • Author(s): 各務 秀明
  • Organizer: 日本再生歯科医学会 シンポジウム 自己骨髄間質細 胞を用いた歯槽骨再生治療
  • Place of Presentation: 鶴見大学会館 メインホール
  • Year and Date: 2010-12-11
• [Book] Heterogeneous responses of human bone marrow stromal cells (multipotent mesenchyme stromal cells) to osteogenic induction. Ed. Hayat MA, Stem Cells and (2011)
  • Author(s): Kagami H, Agata H, Sumita Y, Tojo A.
  • Publisher: Cancer Stem Cells: Therapeutic Applications in Disease and Injury, Volume2, Springer
• [Book] Fundamental technological developments required for increased availability of tissue engineering. (2011)
  • Author(s): Kagami H, Agata H, Kato R, Matsuoka F, Tojo A.
  • Publisher: Ed. Regenerative Medicine and Tissue Engineering: From Cells to Organs. Intech
• [Book] Considerations on designing scaffold for soft and hard tissue engineering. Ed. Gilson Khang. (2011)
  • Author(s): Kagami H, Agata H, Satake M, Narita Y.
  • Publisher: The Handbook of Intelligent Scaffold for Regenerative Medicine Pan Stanford Publishing