2014 Fiscal Year Final Research Report
Understanding mechanisms of evolution of chordates through genome editing technologies
| Project/Area Number |
23681039
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | 脊索動物 / 進化 / 遺伝子改変 / ノックアウト / カタユウレイボヤ / 脊椎動物 |
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| Outline of Final Research Achievements |
This study aimed to establish molecular and genetic technologies for investigating gene functions in the chordate Ciona intestinalis for elucidating mechanisms of the evolution of chordates. Through transposon-mediated transgenesis and gene knockouts, this study revealed functions of various genes that are conserved among chordates in this ascidian. Hox10 is essential for forming the intestine by regulating the migration of endodermal cells. Gonadotropin-releasing hormones expressed at the larval stage are involved in the regulation of metamorphosis of Ciona. New mechanisms of cell cycle regulation has been revealed that are mediated by conserved, developmentally relevant transcription factors. These new findings provide us new insights for deepen the knowledge about the evolution of chordates and ascidians.
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| Free Research Field |
発生遺伝学
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