2012 Fiscal Year Final Research Report
Genomic targets of Mecp2 and Foxg1 in mouse visual cortex
| Project/Area Number |
23681041
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical genome science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ARNER Erik 独立行政法人理化学研究所, LSA 情報基盤施設, 研究員 (20571839)
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| Research Collaborator |
CARNINCI Piero 独立行政法人理化学研究所, ゲノム機能研究チーム, チームリーダー (10333296)
FORREST Alistair 独立行政法人理化学研究所, LSA 情報基盤施設, 施設長 (40569084)
SAXENA Alka 独立行政法人理化学研究所, ゲノム機能研究チーム, 上級研究員 (20618772)
FAGIOLINI Michela Children's Hospital Boston, Assistant Professor of Neurology
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| Project Period (FY) |
2011 – 2012
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| Keywords | Rett Syndrome / Mecp2 / FoxG1 / ChIP Sequencing / Transcriptome Analysis |
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| Research Abstract |
In a mouse model of Rett Syndrome, ChIP-sequencing was used to identify targets of Mecp2 and Foxg1 in visual cortex of wild-type (WT) and Mecp2 knockout (KO) mice. Cap Analysis of Gene Expression (CAGE) to assess the expression levels of these targets in Mecp2 null mice. Differentially expressed genes between WT and KO were mostly observed at a late stage of development, and were enriched for mitochondrial processes including the respiratory chain, suggesting a mitochondrial component to Rett Syndrome.
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