2013 Fiscal Year Final Research Report
Elucidation of novel innate immune system by analysis of host-parasite interaction
| Project/Area Number |
23689029
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Keywords | インターフェロンγ / 自然免疫 / トキソプラズマ原虫 |
|---|
| Research Abstract |
Interferon-g (IFN-g) is essential for host defense against intracellular pathogens. Stimulation of innate immune cells by IFN-g up-regulates ~2000 effector genes such as immunity-related GTPases including p65 guanylate-binding protein (GBP) family genes. We show that a cluster of GBP genes is required for host cellular immunity against the intracellular parasite Toxoplasma gondii. We generated mice deficient for all six GBP genes located on chromosome 3 by targeted chromosome engineering. Mice lacking Gbpchr3 were highly susceptible to T. gondii infection, resulting in increased parasite burden in immune organs. Furthermore, Gbpchr3-deleted macrophages were defective in IFN-g-mediated suppression of T. gondii intracellular growth. In addition, some members of Gbpchr3 restored the protective response against T. gondii in Gbpchr3-deleted cells. Thus, Gbpchr3 redundantly play a pivotal role in anti-T. gondii host defense by controlling IFN-g-mediated cellular innate immunity.
|
-
[Journal Article] Role of the mouse and human autophagy proteins in IFN-γ-induced cell-autonomous responses against Toxoplasma gondii2014
Author(s)
Ohshima J, Lee Y, Sasai M, Saitoh T, Ma JS, Kamiyama N, Matsuura Y, Pann-Ghill S, Hayashi M, Ebisu S, Takeda K, Akira S, Yamamoto M
-
Journal Title
J Immunol
Volume: 192
Pages: 3328-3335
DOI
Peer Reviewed
-
[Journal Article] A cluster of interferon-γ-inducible p65 GTPases plays a critical role in host defense against Toxoplasma gondii2012
Author(s)
Yamamoto M, Okuyama M, Ma JS, Kimura T, Kamiyama N, Saiga H, Ohshima J, Sasai M, Kayama H, Okamoto T, Huang DS, Soldati-Favre D, Horie K, Takeda J, Takeda K
-
Journal Title
Immunity
Volume: 37
Pages: 302-313
Peer Reviewed
-
[Journal Article] ATF6βis a host cellular target of the Toxoplasma gondii virulence factor ROP182011
Author(s)
Yamamoto M, Ma JS, Mueller C, Kamiyama N, Saiga H, Kubo E, Kimura T, Okamoto T, Okuyama M, Kayama H, Nagamune K, Takashima S, Matsuura Y, Soldati-Favre D, Takeda K
-
Journal Title
J Exp Med
Volume: 208
Pages: 1533-1546
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
[Presentation] Critical function of a Cluster of IFN-γ-indudible p65 GTPases in Host Defense Against Toxoplasma gondii2012
Author(s)
Yamamoto M, Okuyama M, Ma JS, Kamiyama N, Ohshima J, Soldati-Favre D, Takeda J, Takeda K
Organizer
WHIP 2012 16^<th> Annual Woods Hole ImmunoParasitology Conference
Place of Presentation
Marine Biological Laboratory, Woods Hole, MA, USA
Year and Date
20120422-25
-
-
-
-
