2012 Fiscal Year Final Research Report
Mechanism of oriented-axon outgrowth in developmentalbrain
| Project/Area Number |
23700448
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
TORIYAMA Michinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (90457151)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脳・神経 / 発生・分化 / 神経科学 / 細胞極性 / 軸索ガイダンス / 軸索伸長 / shootin1 / netrin-1 |
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| Research Abstract |
Here we show that shootin1 is located at a critical interface, transducing a chemical signal into traction forces for axon outgrowth. We found that a netrin-1 positively regulates Pak1-mediated shootin1 phosphorylation. This phosphorylation enhanced the interaction between shootin1 and F-actin retrograde flow, thereby promoting F-actin-substrate coupling, force generation, and axon outgrowth. These results suggest that dynamic actin-substrate coupling can transduce chemical signals into mechanical forces to control cellular motility and provide a molecular-level description of how this transduction may occur.
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