Analysis of glycan-related gene manipulated mice as new animal models of mental disease

2013 Fiscal Year Final Research Report

• Project/Area Number: 23700509
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kanazawa University
• Principal Investigator: YOSHIHARA Toru 金沢大学, 子どものこころの発達研究センター, 特任助教 (00401935)
• Project Period (FY): 2011 – 2013
• Keywords: ノックアウトマウス / 糖鎖 / 精神疾患 / 糖転移酵素 / 行動解析

Research Abstract

In this study, we focused on the functions of glycosyltransferases that acts as key factors in behavioral regulators. To reveal the relationships between those glycosyltransferases and human mental / neurological disease, we employed several strains of gene mamipulated mice and behavioral, histological and biochemical strategies were used. As result, 1) the knockout mouse related to the glycoprotein formation showed enhanced anxiety and disturbance of attention / sensory gating. 2) the knockout mouse related to the glycolipid formation showed severe motor disfunction and developmental retardation that could be explained by axonal disorganization. Therefore these behavioral phenotypes observed in several gene manipulated mice have similarities in human mental / neurological disease, we could confirm that these model mice are useful for the understanding of human disease.

Research Products

• [Journal Article] Anxiety-and depression-like behavior in mice lacking the CD157/BST1 gene, a risk factor for Parkinson's disease (2014)
  • Author(s): Lopatina, O., Yoshihara, T., Nishimura, T., Zhong, J., Akther, S., A. K. M. Fakhrul, A., Liang, M., Higashida, C., Sumi, K., Furuhara, K., Inahata, Y., Huang, J. J., Koizumi, K., Yokoyama, S., Tsuji, T., Petugina, Y., Sumarokov, A., Salmina, A. B., Hashida. K., Kitao. Y., Hori, O., Asano, M., Kitamura, Y., Kozaka, T., Shiba, K., Zhong, F., Xie, M., Sato, M., Ishihara, K. and Higashida, H.
  • Journal Title: Frontiers in Behavioral Neuroscience Volume: 8 Pages: 13
  • DOI: 10.3389/fnbeh.2014.00133
  • Peer Reviewed
• [Journal Article] Displays of paternal parental behaviour in mice following communicative interaction with the maternal mate (2013)
  • Author(s): Liu, H.X., Lopatina, O., Higashida, C., Fujimoto, H., Akther, S., Inzhutova, A., Liang, M., Zhong. J., Tsuji, T., Yoshihara, T., Sumi, K., Ishiyama, M., Ozaki, M., Yagitani, S., Yokoyama, S., Mukaida, N., Sakurai, T., Hori, O., Yoshioka, T., Hirao, A., Kato, Y., Ishihara, K., Kato, I., Okamoto, H., Hirai, H., Asano, M., Brown, D.A., Nagano, I., Higashida,.H.
  • Journal Title: Nature Communications Volume: 4
  • DOI: 10.1038/ncomms2336
  • Peer Reviewed
• [Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice (2012)
  • Author(s): Ito, M., Sugihara, K., Asaka, T., Toyama, T., Yoshihara, T., Furuichi, K., Wada, T. and Asano, M.
  • Journal Title: PLoS ONE Volume: 7 Pages: e29873
  • DOI: 10.1371/journal.pone.0029873
  • Peer Reviewed
• [Journal Article] Specific Enzyme Complex of .-1,4-Galactosyltransferase-II and Glucuronyltransferase-P Facilitates Biosynthesis of N-linked Human Natural Killer-1 (HNK-1) Carbohydrate (2011)
  • Author(s): Kouno, T., Kizuka, Y., Nakagawa. N., Yoshihara. T., Asano, M. and Oka, S.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 31337-31346
  • DOI: 10.1074/jbc.M111.233353
  • Peer Reviewed
• [Presentation] ラクトシルセラミド合成に関わるガラクトース転移酵素 β4GalT-5と-6欠損マウスの行動解析を中心とした脳・神経機能の解析 (2013)
  • Author(s): 吉原亨・佐武寛之・鈴木紘史・西江敏和・引持陽子・神村栄吉・沖野望・伊東信・古川鋼一・浅野雅秀
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2013-12-05
• [Presentation] ラクトシルセラミド合成に関わるガラクトース転移酵素群欠損マウスの行動解析と脳の組織学的・生化学的解析 (2013)
  • Author(s): 吉原亨・佐武寛之・鈴木紘史・西江敏和・引持陽子・神村栄吉・沖野望・伊東信・古川鋼一・浅野雅秀
  • Organizer: 第73回日本動物心理学会大会
  • Place of Presentation: 筑波大学
  • Year and Date: 2013-09-13
• [Presentation] ラクトシルセラミド合成に関わるガラクトース転移酵素群欠損マウスの行動解析と脳の組織学的・生化学的解析 (2013)
  • Author(s): 吉原亨・佐武寛之・鈴木紘史・西江敏和・引持陽子・神村栄吉・沖野望・伊東信・古川鋼一・浅野雅秀
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2013-05-16
• [Presentation] Reduction of lactosylceramide synthase activity and behavioral abnormalities in brain-specific β -galactosyltransferase-5-deficient mouse (2012)
  • Author(s): Yoshihara, T., Suzuki, H., Nishie, T., Hikimochi, Y., Kamimura, E., Okino, N., Ito, M. and Asano, M.
  • Organizer: The 8th FENS Forum of Neuroscience
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2012-07-15
• [Presentation] 脳・神経系特異的β-1,4-ガラクトース転移酵素-5欠損マウスにおける活動性・注意機能の障害 (2012)
  • Author(s): 吉原亨,鈴木紘史,西江敏和,引持陽子,神村栄吉,沖野望,伊東信,浅野雅秀
  • Organizer: 第59回日本実験動物学会総会
  • Place of Presentation: 別府コンベンションセンター
  • Year and Date: 2012-05-25
• [Presentation] Analysis of brain function in neuron specific beta4-galactosyltransferase-5 deficient mouse by using behavioral test-battery (2011)
  • Author(s): 鈴木紘史,吉原亨,西江敏和,引持陽子,神村栄吉,浅野雅秀
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14