2012 Fiscal Year Final Research Report
Analysis of In Situ Chemical Reaction Dynamics by a Multiple Resonance NMR Technique
| Project/Area Number |
23710268
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMADA Hisatsugu 京都大学, 先端医工学研究ユニット, 助教 (80512764)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞内化学反応 / 多重共鳴 NMR / 分子プローブ / NMR イメージング |
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| Research Abstract |
NMR/MR is one of the most promising techniques for the in situ analysis of specific biochemical (metabolic) reactions, both in vitro and in vivo. In this research, we revealed that (1) one-dimensional triple-resonance NMR is applicable to metabolic analysis of 13C/15N-labeled uracil, (2) integration of stable isotopes into the biocompatible polymer-tag (13C/15N-PMPC) enabled observation of the selective triple resonance NMR signal of 13C/15N-PMPC at a nano-molar level in a mouse liver lysate, and (3) application of a multiple resonance NMR technique to a MR imaging allows us to obtain the selective MR image of 13C/15N-PMPC without endogenous noise signal.
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[Journal Article] Substrate/Product-Targeted NMR Monitoring of Pyrimidine Catabolism and Its Inhibition by a Clinical Drug2012
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S
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Journal Title
ACS Chem. Biol.
Volume: 7
Pages: 535-542
DOI
Peer Reviewed
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[Presentation] Triple resonance NMR in application to in situ monitoring of targeted metabolic reaction and its inhibition by a clinical drug2012
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S.
Organizer
244th ACS National Meeting
Place of Presentation
Philadelphia, PA
Year and Date
2012-08-21
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[Presentation] Direct In Situ Monitoring of Pyrimidine Catabolism and Its Inhibition Using Triple Resonance NMR Analysis2011
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S
Organizer
38thInternational Symposium on Nucleic Acids Chemistry
Place of Presentation
Hokkaido Univ., Sapporo, Japan
Year and Date
2011-11-10
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