2013 Fiscal Year Final Research Report
Development of High Performance Enzyme Activity Assay for Glycoside Hydrolase Using Metal-ions as Indicator
| Project/Area Number |
23750075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukushima University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 酵素活性測定 / バイオマス / セルロース / セルラーゼ |
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| Research Abstract |
In this study, the simple determination method of cellulase has been developed using copper(II)-phthalocyanine complex modified cellulose with the alternative of an inductively coupled plasma -optical emission spectrometry (ICP-OES) or flame-less atomic absorbance spectrometer(FL-AAS). Then, although the cellulase does not include the metal ion in the molecule, the metal ion is released by the biodigestion of copper(II)-phthalocyanine complex modified cellulose into the solution. The presented method can measure the cellulase concentration and the activity in short time (within 15min) as comparing with common spectrophotometric method.
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Research Products
(13 results)
