2012 Fiscal Year Final Research Report
X-ray crystallography of abscisic acid transporter
| Project/Area Number |
23770104
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIYAKAWA Takuya 東京大学, 大学院・農学生命科学研究科, 助教 (50596559)
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| Project Period (FY) |
2011 – 2012
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| Keywords | X線結晶解析 |
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| Research Abstract |
This research has established a method for preparing two types of abscisic acid (ABA) transporters that are key membrane proteins for an intercellular ABA transport. The ABA transporters were overexpressed as membrane fractions by using the yeast expression system of Pichia pastoris. Moreover, detergent screening experiments also showed that ABA transporters were efficiently solubilized with Fos-choline 12 or LDAO among several tested detergents. These results are available with sample preparation for the structural analysis of the ABA transporters, which helps us understand the molecular mechanism of intercellular ABA transport.
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