2012 Fiscal Year Final Research Report
Role of FBP17,an EFC/F-BAR protein, in phagocytic cupformation
| Project/Area Number |
23770228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kobe University |
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Principal Investigator |
TSUJITA Kazuya 神戸大学, 大学院・医学研究科, 助教 (10457054)
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| Project Period (FY) |
2011 – 2012
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| Keywords | F-BAR / ドメイン / アクチン重合 |
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| Research Abstract |
By performing in vitro analysis, we directly showed the mechanism by which FBP17 regulates actin polymerization on the membrane tubules. Physiologically, it was found that FBP17 is recruited to phagocytic cup via its F-BAR domain and that this accumulation is critical for phagocytic cup formation. Furthermore, RNAi and its rescue experiments revealed that the assembly of FBP17 at the plasma membrane is dependent on its F-BAR domain, promoting actin polymerization at phagocytic cup membrane. These data suggest that invaginated membrane caused by FBP17 may function as a scaffold for actin polymerization.
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