2013 Fiscal Year Final Research Report
Regulatory mechanism of the periodic expression of Ripply3 during pharyngeal arch segmentation
| Project/Area Number |
23770268
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 咽頭弓分節 / 転写制御 / Tbx1 / Ripply3 |
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| Research Abstract |
Pharyngeal arch (PA) is a transient segmental structure appears in the anterior part of vertebrate embryos. Appropriate PA segmentation is required for the normal organogenesis such as heart, thymus, and others at later stages. We recently identified the Ripply3 gene that is highly expressed in the developing PA. Interestingly, the prominent expression emerges in the unsegmented PA region first, and then it decreases as PA segmentation proceeds. Thus, Ripply3 expression shifts periodically from anterior to posterior. To elucidate the molecular mechanism of the unique expression of Ripply3 during PA segmentation, we generated the EGFP reporter transgenic mouse driven by Ripply3 promoter. The EGFP expression recapitulated the endogenous expression pattern of Ripply3. Furthermore, the promoter is activated by Tbx1, and repressed by Ripply3 itself in vitro. These studies revealed that Tbx1 and Ripply3 have important roles for the periodic expression of Ripply3.
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Research Products
(8 results)
