2012 Fiscal Year Final Research Report
Functional analysis of a novel phosphorylation regulator in MAMPs signaling
Project/Area Number |
23780048
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plant pathology
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
MATSUI Hidenori 独立行政法人理化学研究所, 植物プロテオミクス研究ユニット, 特別研究員 (20598833)
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Project Period (FY) |
2011 – 2012
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Keywords | 植物免疫 / プロテオミクス / リン酸化 / シグナル伝達 |
Research Abstract |
To identify the phosphorylation signal network in plant immunity, we performed screening of T-DNA insertion lines in Arabidopsis to determine proteins with changed phosphorylation status in response to MAMP treatment as indicated by measurement of reactive oxygen species (ROS) by flg22 treatment. We identified 38 genes that showed abnormal ROS production after flg22 treatment. Additionally, some candidates exhibited the abnormal immune responses against bacterial pathogen infection, suggesting that phosphoproteomic approaches have the potential to identify novel immune signaling components in plants.
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