Degradation of b-glucan absorbed from intestine and the effect of degraded b-glucan on the intestinal immune function

2013 Fiscal Year Final Research Report

• Project/Area Number: 23780131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Shizuoka University
• Principal Investigator: HINO Shingo 静岡大学, (連合)農学研究科(研究院), 助教 (70547025)
• Project Period (FY): 2011 – 2013
• Keywords: β-グルカン / マクロファージ / 活性酸素

Research Abstract

Using the extracellular domain of Dectin-1 as b-glucan-specific probes, we succeeded in detection of soluble and Dectin-1-reactive b-glucan discharged from macrophage cell lines as well as mouse peritoneal macrophages, which had phagocytized insoluble b-glucan particles. The RAW264.7 cell culture-supernatant containing the discharged b-glucan stimulated RAW264.7 cells, resulting in the induction of cytokine expression. Such discharge of Dectin-1-reactive b-glucan from macrophage cells was inhibited by either NADPH oxidase inhibitors or radical scavengers. These results suggest that degraded but Dectin-1-reactive b-glucan is discharged from macrophage cells phagocytizing insoluble b-glucan particles and stimulates the other phagocytes.

Research Products

• [Journal Article] Measurement of barleyβ-glucan concentration in the plasma by sandwich ELISA using rat dectin-1 (2013)
  • Author(s): Hino, S., Sato, H., Matsuda, T., Morita, T
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 77(2) Pages: 413-415
  • DOI: 10.1271/bbb.120788
  • Peer Reviewed
• [Journal Article] Discharge of solubilized and Dectin-1-reactiveβ-glucan from macrophage cells phagocytizing insolubleβ-glucan particles (2012)
  • Author(s): Hino, S., Kito, A., Yokoshima, R., Sugino, R., Oshima, K., Morita, T., Okajima, T., Nadano, D., Uchida, K., Matsuda, T
  • Journal Title: Involvement of reactive oxygen species (ROS)-driven degradation、Biochem. Biophys. Res. Commun Volume: 421 Pages: 329-334
  • DOI: 10.1016/j.bbrc.2012.04.009
• [Journal Article] Small intestinal goblet cell proliferation induced by ingestion of soluble and insoluble dietary fiber is characterized by an increase in sialylated mucins in rats (2012)
  • Author(s): Hino, S., Takemura, N., Sonoyama, K., Morita, A., Kawagishi, H., Aoe, S., Morita, T
  • Journal Title: J. Nutr Volume: 142 Pages: 1429-1436
  • DOI: 10.3945/jn.112.159731
• [Presentation] β-Glucanの免疫修飾作用と腸管吸収 (2012)
  • Author(s): 日野真吾
  • Organizer: シーズ&ニーズビジネスマッチング研究発表会-高齢化社会・健康・食品産業-
  • Place of Presentation: ホテルアソシア静岡
  • Year and Date: 20121200
• [Presentation] 消化管におけるβ-glucanの上皮細胞およびマクロファージによる認識 (2012)
  • Author(s): 日野真吾
  • Organizer: 第11回Hindgut Club Japanサテライトミーティング(第66回日本栄養・食糧学会大会)
  • Place of Presentation: 東北大学
  • Year and Date: 20120500
• [Presentation] 消化管におけるβ-glucanの上皮細胞およびマクロファージによる認識 (2011)
  • Author(s): 日野真吾
  • Organizer: 第17回Hindgut Club JAPANシンポジウム
  • Place of Presentation: 専修大学
  • Year and Date: 20111200
• [Presentation] β-グルカンの腸管からの吸収と分解機構 (2011)
  • Author(s): 日野真吾
  • Organizer: 日本農芸化学会中部支部第163回例会
  • Place of Presentation: 東海軒会館
  • Year and Date: 20111200
• [Presentation] β-Glucan and Its Implication for Health Benefits (2011)
  • Author(s): Hino S
  • Organizer: where is it incorporated from?、2nd Korea-Japan Joint Symposium and Graduate Students Forum
  • Place of Presentation: Kyungpook National University (Korea)
  • Year and Date: 20110900
• [Remarks]
  • URL: http://www.agr.shizuoka.ac.jp/abc/morita_t/index.html