A omputational study on the intermolecular interaction between influenza virus hemagglutinin and a monoclonal antibody

2013 Fiscal Year Final Research Report

• Project/Area Number: 23780305
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: IGARASHI Manabu 北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (10374240)
• Project Period (FY): 2011 – 2012
• Keywords: インフルエンザ / ウイルス / 計算科学 / 分子動力学 / 抗体

Research Abstract

Influenza A virus is a zoonotic pathogen that is transmitted from animals to humans. This virus has two surface glycoproteins, hemagglutinin (HA) and neuraminidase. The HA of human influenza virus is the major target of antibodies that neutralize viral infectivity. Some amino acid substitutions in the antigenic epitope on the HA could decrease the interaction between HA and antibodies, leading to generate the antigenic variants with a novel antigenic structure of HA. To understand how amino acid substitutions affect the changes of the HA antigenic structure, we computationally analyzed the three-dimensional structures of HA in complex with monoclonal antibody.

Research Products

• [Journal Article] Antiviral activity of stachyflin on influenza A viruses of different hemagglutinin subtypes (2013)
  • Author(s): Motohashi Y, Igarashi M, Okamatsu M, Noshi T, Sakoda Y, Yamamoto N, Ito K, Yoshida R, Kida H
  • Journal Title: Virol J Volume: 10 Pages: 118
  • DOI: 10.1186/1743-422X-10-118
  • Peer Reviewed
• [Journal Article] Resampling nucleotide sequences with closest-neighbor trimming and its comparison to other methods (2013)
  • Author(s): Yonezawa K, Igarashi M, Ueno K, Takada A, Ito K
  • Journal Title: PLoS One Volume: 8 (10) Pages: e57684
  • DOI: 10.1371/journal.pone.0057684
  • Peer Reviewed
• [Journal Article] Novel mutations in Marburg virus glycoprotein associated with viral evasion from antibody-mediated immune pressure (2013)
  • Author(s): Kajihara M, Nakayama E, Marzi A, Igarashi M, Feldmann H, Takada A
  • Journal Title: J Gen Virol Volume: 94(4) Pages: 876-883
  • DOI: 10.1099/vir.0.049114-0
  • Peer Reviewed
• [Journal Article] Molecular mechanisms underlying oseltamivir resistance mediated by an I117V substitution in the neuraminidase of subtype H5N1 avian influenza A viruses (2013)
  • Author(s): Takano R, Kiso M, Igarashi M, Le QM, Sekijima M, Ito K, Takada A, Kawaoka Y
  • Journal Title: J Infect Dis Volume: 207(1) Pages: 88-97
  • DOI: 10.1093/infdis/jis633
  • Peer Reviewed
• [Journal Article] Gnarled-Trunk Evolutionary Model of Influenza A Virus Hemagglutinin (2013)
  • Author(s): Ito K, Igarashi M, Miyazaki Y, Murakami T, Iida S, Kida H, Takada A
  • Journal Title: PLoS One Volume: 6(10) Pages: e25953
  • DOI: 10.1371/journal.pone.0025953
  • Peer Reviewed
• [Journal Article] An H5N1 highly pathogenic avian influenza virus that invaded Japan through waterfowl migration (2013)
  • Author(s): Kajihara M, Matsuno K, Simulundu E, Muramatsu M, Noyori O, Manzoor R, Nakayama E, Igarashi M, Tomabechi D, Yoshida R, Okamatsu M, Sakoda Y, Ito K, Kida H, Takada A
  • Journal Title: Jpn J Vet Res Volume: 59 Pages: 89-100
  • URL: http://hdl.handle.net/2115/47149
  • Peer Reviewed
• [Remarks]
  • URL: http://www.czc.hokudai.ac.jp/bioinform/members/igarashi/index_j.html